Tead1 reciprocally regulates adult β-cell proliferation and function

Jeongkyung Lee, Ruya Liu, Byung S. Kim, Yiqun Zhang, Feng Li, Rajaganapti Jagannathan, Ping Yang, Vinny Negi, Eliana Melissa Perez-Garcia, Pradip K. Saha, Omaima Sabek, Cristian Coarfa, Chad J. Creighton, Mark O. Huising, Rita Bottino, Ke Ma, Mousumi Moulik, Vijay K. Yechoor

Research output: Contribution to journalArticle

Abstract

Diabetes ensues when there is a net decrease in functional β-cell mass. Efforts to increase β-cell mass are limited by a concurrent loss of mature function. The molecular mechanisms underlying the reciprocal regulation between β-cell proliferation and mature function are unclear. Here, we demonstrate that constitutive and inducible genetic deletion of Tead1 in mouse β-cells leads to diabetes. Tead1, the transcription factor downstream of the mammalian-hippo pathway, has a developmental stage-specific critical function in β-cells and is required for maintenance of mature β-cell functional competence by direct transcriptional regulation of a network of critical β-cell transcription factors, including, Pdx1, Nkx6.1 and MafA. Concurrently, Tead1 directly activates Cdkn2a transcription in adult β-cells to inhibit proliferation. Studies in human β-cells demonstrate that Tead1 plays a similar regulatory role. Taken together, our findings uncover the non-redundant role of Tead1 in modulating the balance between proliferative capacity and functional competence.
Original languageEnglish (US)
JournalbioRxiv
DOIs
StateUnpublished - Mar 5 2020

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