T‐Cell Stimulation Induced by Idiotypes on Monoclonal Immunoglobulins in Patients with Monoclonal Gammopathies

Qing Yi, S. BERGENBRANT, A. ÖSTERBORG, E. ÖSBY, R. ÖSTMAN, M. BJÖRKHOLM, G. HOLM, A. K. LEFVERT

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

The stimulation of peripheral blood mononuclear cells by purified autologous and/or allogeneic monoclonal IgG was studied in five patients with multiple myeloma (MM), nine patients with monoclonal gammopathy of undetermined significance (MGUS) and six healthy individuals. Single cells secreting IFN‐γ or IL‐2 were identified using an enzyme‐linked immunospot assay. Patients' cells were preferentially stimulated by autologous monoclonal IgG at low concentrations (1–100 pg/ml). while l00 ng/ml or higher stimulated T cells both from patients and, to a lesser degree, healthy individuals. This biphasic dose‐response of T‐cell stimulation by autologous monoclonal IgG was reproduced in all patients. The numbers of cells secreting IFN‐γ and IL‐2 in response to allogeneic IgG were significantly lower than the numbers obtained using autologous IgG in patients with MM and MGUS. Cells from healthy individuals were stimulated by allogeneic monoclonal IgG, but to a lesser extent. The results of this study support the presence of idiotype‐reactive T cells in patients with MM and MGUS and also may suggest a general but less pronounced T‐cell reactivity to monoclonal IgG among these patients.

Original languageEnglish (US)
Pages (from-to)529-534
Number of pages6
JournalScandinavian Journal of Immunology
Volume38
Issue number6
DOIs
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Immunology

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