TBKBP1 and TBK1 form a growth factor signalling axis mediating immunosuppression and tumourigenesis

Lele Zhu, Yanchuan Li, Xiaoping Xie, Xiaofei Zhou, Meidi Gu, Zuliang Jie, Chun Jung Ko, Tianxiao Gao, Blanca E. Hernandez, Xuhong Cheng, Shao Cong Sun

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

TANK-binding kinase 1 (TBK1) responds to microbial stimuli and mediates the induction of type I interferon (IFN). Here, we show that TBK1 is also a central mediator of growth factor signalling; this function of TBK1 relies on a specific adaptor—TBK-binding protein 1 (TBKBP1). TBKBP1 recruits TBK1 to protein kinase C-theta (PKCθ) through a scaffold protein, CARD10. This enables PKCθ to phosphorylate TBK1 at Ser 716, a crucial step for TBK1 activation by growth factors but not by innate immune stimuli. Although the TBK1–TBKBP1 signalling axis is not required for the induction of type I IFN, it mediates mTORC1 activation and oncogenesis. Conditional deletion of either TBK1 or TBKBP1 in lung epithelial cells inhibits tumourigenesis in a mouse model of lung cancer. In addition to promoting tumour growth, the TBK1–TBKBP1 axis facilitates tumour-mediated immunosuppression through a mechanism that involves induction of the checkpoint molecule PD-L1 and stimulation of glycolysis. These findings suggest a PKCθ–TBKBP1–TBK1 growth factor signalling axis that mediates both tumour growth and immunosuppression.

Original languageEnglish (US)
Pages (from-to)1604-1614
Number of pages11
JournalNature Cell Biology
Volume21
Issue number12
DOIs
StatePublished - Dec 1 2019

ASJC Scopus subject areas

  • Cell Biology

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