Taxol induces internucleosomal DNA fragmentation associated with programmed cell death in human myeloid leukemia cells

Kapil Bhalla, Ana Maria Ibrado, Elena Tourkina, Caroline Tang, Mary Ella Mahoney, Yue Huang

Research output: Contribution to journalArticle

326 Scopus citations

Abstract

The present results demonstrate that the exposure of human myeloid leukemia HL-60 and KG-1 cells to clinically achievable concentrations of taxol produced internucleosomal DNA fragmentation of approximately 200 base-pair multiples, and the morphologic changes characteristic of cells undergoing programmed cell death (PCD) or apoptosis. Taxol-induced PCD was associated with a marked inhibition of suspension culture growth and clonogenic survival of HL-60 cells. In addition, taxol treatment decreased BCL-2 oncogene expression, which is known to block PCD. The exposure to taxol moderately decreased c-myc expression, but did not induce c-jun expression - which has been previously noted for a variety of DNA interactive, antileukemic drugs. These findings indicate that taxol may induce leukemic cell death partly by the alternative but gene-directed and active mechanism of PCD.

Original languageEnglish (US)
Pages (from-to)563-568
Number of pages6
JournalLeukemia
Volume7
Issue number4
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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