T cells with specificity for idiotypic determinants on human monoclonal autoantibodies in myasthenia gravis

Q Yi, R Ahlberg, A K Lefvert

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The activation of T cells from 46 patients with myasthenia gravis and 28 healthy individuals by two human monoclonal autoantibodies was studied. B-cell clones were produced by transformation of peripheral lymphocytes from a patient using Epstein-Barr virus and subsequent cloning. Two myasthenia-specific autoantibodies, one anti-receptor-antibody and one antiidiotypic antibody, both carrying separate recurrent idiotopes, were used in this study. Single activated T cells were identified by their secretion of IL2 and IFN gamma using a cell enzyme-linked-immunosorbent technique. The idiotypic antibody activated T cells in patients but not in most of the controls at concentrations of 1 pg/ml and 10 pg/ml. High concentrations of antibody resulted in T-cell activation in both groups. A similar dose-response pattern was recorded using the antiidiotypic antibody. Incubation with the idiotypic antibody resulted in T-cell stimulation, measured as numbers of IFN gamma-secreting cells that exceeded the mean +2 SD of controls, in 78% of patients and in 7% of the healthy individuals (p less than 0.001). The antiidiotypic antibody activated T cells in 50% of patients and in 4% of the healthy individuals (p less than 0.001). T-cell activation measured as numbers of IL2-secreting cells showed a difference between patients and controls which was as significant as for IFN gamma secretion. The results demonstrate the presence of T cells with specificity for disease-specific determinants on idiotypic and antiidiotypic antibodies in myasthenia gravis.

Original languageEnglish (US)
Pages (from-to)149-56
Number of pages8
JournalJournal of clinical & cellular immunology
Volume143
Issue number2
StatePublished - Feb 1992

Keywords

  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Monoclonal
  • Autoantibodies
  • Female
  • Humans
  • Immunoglobulin Idiotypes
  • In Vitro Techniques
  • Interferon-gamma/biosynthesis
  • Interleukin-2/biosynthesis
  • Lymphocyte Activation
  • Male
  • Middle Aged
  • Myasthenia Gravis/immunology
  • T-Lymphocytes/immunology

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