T-cell help in human in vitro antibody-producing systems: Role of inhibitory T cells in masking allogeneic help

M. K. Brenner, A. J. Munro

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A system is described for specific antibody production from human peripheral blood lymphocytes, using tetanus toxoid as antigen and a solid-phase radioimmunoassay to measure antibody. Culture in human sera during the induction phase of antibody formation may lead to cell pickup of AgAb complexes, detectable as "secreted" antibody. This problem can be overcome by depleting adherent cells, thereby enhancing the culture system's antigen sensitivity. In this system unirradiated T cells do not cooperate with allogeneic B cells. This lack of cooperation represents allogeneic inhibitory effects of a radiation-sensitive T cell, for irradiation permits detection of apparently unrestricted T-cell help.

Original languageEnglish (US)
Pages (from-to)201-208
Number of pages8
JournalCellular Immunology
Volume57
Issue number1
DOIs
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Immunology

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