A system is described for specific antibody production from human peripheral blood lymphocytes, using tetanus toxoid as antigen and a solid-phase radioimmunoassay to measure antibody. Culture in human sera during the induction phase of antibody formation may lead to cell pickup of AgAb complexes, detectable as "secreted" antibody. This problem can be overcome by depleting adherent cells, thereby enhancing the culture system's antigen sensitivity. In this system unirradiated T cells do not cooperate with allogeneic B cells. This lack of cooperation represents allogeneic inhibitory effects of a radiation-sensitive T cell, for irradiation permits detection of apparently unrestricted T-cell help.
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