T cell exhaustion is associated with antigen abundance and promotes transplant acceptance

Dawei Zou, Yulin Dai, Xiaolong Zhang, Guohua Wang, Xiang Xiao, Peilin Jia, Xian C. Li, Zhiyong Guo, Wenhao Chen

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Exhaustion of T cells limits their ability to clear chronic infections or eradicate tumors. Here, in the context of transplant, we investigated whether T cell exhaustion occurs and has a role in determining transplant outcome. A peptide/MHC tetramer-based approach was used to track exhausted CD8+ T cells in a male-to-female skin transplant model. Transplant of large whole-tail skins, but not small tail skins (0.8 cm × 0.8 cm), led to exhaustion of anti-male tetramer+ CD8+ T cells and subsequently the acceptance of skin grafts. To study CD4+ T cell exhaustion, we used the TCR-transgenic B6 TEa cells that recognize a major transplant antigen I-Eα from Balb/c mice. TEa cells were adoptively transferred either into B6 recipients that received Balb/c donor skins or into CB6F1 mice that contained an excessive amount of I-Eα antigen. Adoptively transferred TEa cells in skin-graft recipients were not exhausted. By contrast, virtually all adoptively transferred TEa cells were exhausted in CB6F1 mice. Those exhausted TEa cells lost ability to reject Balb/c skins upon further transfer into lymphopenic B6.Rag1−/− mice. Hence, T cell exhaustion develops in the presence of abundant antigen and promotes transplant acceptance. These findings are essential for better understanding the nature of transplant tolerance.

Original languageEnglish (US)
Pages (from-to)2540-2550
Number of pages11
JournalAmerican Journal of Transplantation
Volume20
Issue number9
DOIs
StateE-pub ahead of print - Mar 17 2020

Keywords

  • T cell biology
  • basic (laboratory) research / science
  • cellular biology
  • graft survival
  • immunobiology
  • microarray/gene array
  • tolerance: mechanisms

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation
  • Pharmacology (medical)

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