Synthesis of saturated, unsaturated, spin-labeled, and fluorescent cholesteryl esters: Acylation of cholesterol using fatty acid anhydride and 4-pyrrolidinopyridine

K. M. Patel, L. A. Sklar, R. Currie, Henry J. Pownall, J. D. Morrisett, J. T. Sparrow

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

A rapid, high yield method for the preparation of cholesteryl esters is described. The method is a modification of the catalytic procedure previously applied to the acylation of sn-glycero-3-phosphoryl-choline (Patel, K.M., J.D. Morrisett, and J.T. Sparrow, J. Lipid Res., 20:676 (1979). Cholesteryl esters are formed in excellent yield by acylating cholesterol with fatty acid anhydride or fatty acid and dicyclohexylcarbodiimide in methylene chloride containing 4-pyrrolidinopyridine. The versatility of the method is demonstrated by the preparation of the cholesteryl esters of saturated, unsaturated, spinlabeled, and labile fluorescent fatty acids.

Original languageEnglish (US)
Pages (from-to)816-818
Number of pages3
JournalLipids
Volume14
Issue number9
DOIs
StatePublished - Sep 1 1979

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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