Abstract
A rapid, high yield method for the preparation of cholesteryl esters is described. The method is a modification of the catalytic procedure previously applied to the acylation of sn-glycero-3-phosphoryl-choline (Patel, K.M., J.D. Morrisett, and J.T. Sparrow, J. Lipid Res., 20:676 (1979). Cholesteryl esters are formed in excellent yield by acylating cholesterol with fatty acid anhydride or fatty acid and dicyclohexylcarbodiimide in methylene chloride containing 4-pyrrolidinopyridine. The versatility of the method is demonstrated by the preparation of the cholesteryl esters of saturated, unsaturated, spinlabeled, and labile fluorescent fatty acids.
Original language | English (US) |
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Pages (from-to) | 816-818 |
Number of pages | 3 |
Journal | Lipids |
Volume | 14 |
Issue number | 9 |
DOIs | |
State | Published - Sep 1979 |
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry
- Cell Biology