Synergistic interaction between acivicin (AT-125) and 6-thioguanine in the murine leukemia L1210. Biochemical and cytokinetic considerations

Kapil Bhalla, Rabinandreth Nayak, Arlene Deitch, Steven Grant

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The effect of the purine antagonist acivicin (AT-125) on the metabolism and cytotoxicity of 6-thioguanine was examined in the murine leukemia L1210. Cells exposed to 5 × 10-6 M acivicin for 18 hr followed by 10-5 M[14C]-6-thioguanine for 2 hr accumulated 2.90 ± 0.17 nmoles 6-thioguanine/106 cells compared to 0.69 ± 0.07 nmoles 6-thioguanine/106 cells in untreated controls. Intracellular accumulation of 6-thioguanine monophosphate, a lethal 6-thioguanine metabolite, increased from 0.27 ± 0.05 to 1.08 ± 0.13 nmoles 6-thioguanine monophosphate/106 cells following the same acivicin exposure. A similar increment was observed for the formation of 6-thioguanine triphosphate. These alterations in 6-thioguanine metabolism were associated with an increase in the intracellular level of 5-phosphoribosyl-1-pyrophosphate, an obligatory substrate in 6-thioguanine activation (57.9 ± 7.6 vs 13.4 ± 2.3 ng 5-phosphoribosyl-1-pyrophosphate/106 cells). In contrast, there was a 50% reduction in the amount of 6-thioguanine incorporated into RNA and DNA following acivicin pretreatment. Cytofluorometric analysis revealed that an 18-hr exposure to 5 × 106 M acivicin increased the population S-phase cells, which are more sensitive to the actions of 6-thioguanine, by 50% relative to untreated controls. In both suspension culture growth and soft agar studies, the sequential administration of acivicin followed by 6-thioguanine resulted in substantial growth inhibitory activity; in contrast, the effects of the reverse sequence were subadditive. Pretreatment of L1210 cells with acivicin potentiates the action of subsequently administered 6-thioguanine, and the mechanism may involve both biochemical as well as cytokinetic factors. In vivo studies involving the sequential administration of these agents appear warranted.

Original languageEnglish (US)
Pages (from-to)247-254
Number of pages8
JournalBiochemical pharmacology
Volume33
Issue number2
DOIs
StatePublished - Jan 15 1984

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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