Synergistic growth inhibition of anaplastic large cell lymphoma cells by combining cellular ALK gene silencing and a low dose of the kinase inhibitor U0126

M. Ito, N. Zhao, Z. Zeng, C. C. Chang, Y. Zu

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Abnormal expression of anaplastic lymphoma kinase (ALK) gene is an important pathogenic factor for anaplastic large cell lymphoma (ALCL). To study the function of ALK, an inducible short hairpin RNA (shRNA) system was stably introduced into cultured human ALCL cells. Inducing shRNA expression in the generated cells resulted in cellular ALK gene silencing and led to inactivation of multiple signaling pathways and growth arrest. Interestingly, a combination of ALK gene silencing with U0126, a kinase inhibitor specific for the extracellular signal-regulated kinases 1/2 pathway, resulted in an augmented reduction in cellular JunB expression. Functional studies indicated that combining ALK gene silencing with U0126 treatment provided a synergistic growth inhibition, which occurred faster and was more profound than with either treatment alone. This synergistic effect was also observed when measuring cell proliferation, apoptosis, and in vitro cell colony formation. Importantly, the combination of ALK gene silencing and U0126 had a prolonged inhibitory effect, preventing recovery of ALCL cell growth even after treatments were removed. Moreover, this synergistic inhibitory effect was confirmed in vivo using a mouse model with xenografted ALCL tumors. Our findings indicate that combining cellular ALK gene silencing with a low dose of U0126 may prove to be an effective and more specific therapeutic approach to treating ALCL

Original languageEnglish (US)
Pages (from-to)633-644
Number of pages12
JournalCancer Gene Therapy
Volume17
Issue number9
DOIs
StatePublished - Sep 2010

Keywords

  • Anaplastic large cell lymphoma (ALCL)
  • anaplastic lymphoma kinase (ALK)
  • ERK1/2 pathway
  • kinase inhibitor U0126
  • small-interference RNA (siRNA)-induced gene silencing
  • synergistic growth effect

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Molecular Biology

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