Synergism of Rana catesbeiana ribonuclease and IFN-γ triggers distinct death machineries in different human cancer cells

Chih Hang Anthony Tang; Chih Chi Andrew Hu; Chyou Wei Wei; Jaang Jiun Wang

doi:10.1016/j.febslet.2004.11.086

Synergism of Rana catesbeiana ribonuclease and IFN-γ triggers distinct death machineries in different human cancer cells

Chih Hang Anthony Tang, Chih Chi Andrew Hu, Chyou Wei Wei, Jaang Jiun Wang

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Rana catesbeiana ribonuclease (RC-RNase) possesses tumor-specific cytotoxicity, which can be synergized by IFN-γ. However, it is unclear how RC-RNase and RC-RNase/IFN-γ induce cell death. In this study, we use substrate cleavage assays to systematically investigate RC-RNase- and RC-RNase/IFN-γ-induced caspase activation in HL-60, MCF-7, and SK-Hep-1 cells. We find that RC-RNase and RC-RNase/IFN-γ induce mitochondria-mediated caspase activation in HL-60 and MCF-7 cells but not in SK-Hep-1 cells, although death of SK-Hep-1 cells is closely related to mitochondrial disruptions. Our findings provide evidence that RC-RNase and RC-RNase/IFN-γ can kill different cancer cells by distinct mechanisms. Compared with onconase, RC-RNase seems to harbor a more specific anti-cancer activity.

Original language	English (US)
Pages (from-to)	265-270
Number of pages	6
Journal	FEBS Letters
Volume	579
Issue number	1
DOIs	https://doi.org/10.1016/j.febslet.2004.11.086
State	Published - Jan 3 2005

Keywords

Anti-tumor ribonuclease
Caspase
Onconase
Rana catesbeiana ribonuclease

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2004.11.086

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title = "Synergism of Rana catesbeiana ribonuclease and IFN-γ triggers distinct death machineries in different human cancer cells",

abstract = "Rana catesbeiana ribonuclease (RC-RNase) possesses tumor-specific cytotoxicity, which can be synergized by IFN-γ. However, it is unclear how RC-RNase and RC-RNase/IFN-γ induce cell death. In this study, we use substrate cleavage assays to systematically investigate RC-RNase- and RC-RNase/IFN-γ-induced caspase activation in HL-60, MCF-7, and SK-Hep-1 cells. We find that RC-RNase and RC-RNase/IFN-γ induce mitochondria-mediated caspase activation in HL-60 and MCF-7 cells but not in SK-Hep-1 cells, although death of SK-Hep-1 cells is closely related to mitochondrial disruptions. Our findings provide evidence that RC-RNase and RC-RNase/IFN-γ can kill different cancer cells by distinct mechanisms. Compared with onconase, RC-RNase seems to harbor a more specific anti-cancer activity.",

keywords = "Anti-tumor ribonuclease, Caspase, Onconase, Rana catesbeiana ribonuclease",

author = "Tang, {Chih Hang Anthony} and Hu, {Chih Chi Andrew} and Wei, {Chyou Wei} and Wang, {Jaang Jiun}",

note = "Funding Information: The present research was sponsored by grants from the National Science Council, ROC (NSC 92-2320-B-016-028 and NSC 92-2811-B-016-005). The authors are grateful to Dr. Kuslima Shogen (Alfacell Corporation) for her generous supply of onconase.",

year = "2005",

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doi = "10.1016/j.febslet.2004.11.086",

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T1 - Synergism of Rana catesbeiana ribonuclease and IFN-γ triggers distinct death machineries in different human cancer cells

AU - Tang, Chih Hang Anthony

AU - Hu, Chih Chi Andrew

AU - Wei, Chyou Wei

AU - Wang, Jaang Jiun

N1 - Funding Information: The present research was sponsored by grants from the National Science Council, ROC (NSC 92-2320-B-016-028 and NSC 92-2811-B-016-005). The authors are grateful to Dr. Kuslima Shogen (Alfacell Corporation) for her generous supply of onconase.

PY - 2005/1/3

Y1 - 2005/1/3

N2 - Rana catesbeiana ribonuclease (RC-RNase) possesses tumor-specific cytotoxicity, which can be synergized by IFN-γ. However, it is unclear how RC-RNase and RC-RNase/IFN-γ induce cell death. In this study, we use substrate cleavage assays to systematically investigate RC-RNase- and RC-RNase/IFN-γ-induced caspase activation in HL-60, MCF-7, and SK-Hep-1 cells. We find that RC-RNase and RC-RNase/IFN-γ induce mitochondria-mediated caspase activation in HL-60 and MCF-7 cells but not in SK-Hep-1 cells, although death of SK-Hep-1 cells is closely related to mitochondrial disruptions. Our findings provide evidence that RC-RNase and RC-RNase/IFN-γ can kill different cancer cells by distinct mechanisms. Compared with onconase, RC-RNase seems to harbor a more specific anti-cancer activity.

AB - Rana catesbeiana ribonuclease (RC-RNase) possesses tumor-specific cytotoxicity, which can be synergized by IFN-γ. However, it is unclear how RC-RNase and RC-RNase/IFN-γ induce cell death. In this study, we use substrate cleavage assays to systematically investigate RC-RNase- and RC-RNase/IFN-γ-induced caspase activation in HL-60, MCF-7, and SK-Hep-1 cells. We find that RC-RNase and RC-RNase/IFN-γ induce mitochondria-mediated caspase activation in HL-60 and MCF-7 cells but not in SK-Hep-1 cells, although death of SK-Hep-1 cells is closely related to mitochondrial disruptions. Our findings provide evidence that RC-RNase and RC-RNase/IFN-γ can kill different cancer cells by distinct mechanisms. Compared with onconase, RC-RNase seems to harbor a more specific anti-cancer activity.

KW - Anti-tumor ribonuclease

KW - Caspase

KW - Onconase

KW - Rana catesbeiana ribonuclease

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Synergism of Rana catesbeiana ribonuclease and IFN-γ triggers distinct death machineries in different human cancer cells

Abstract

Keywords

ASJC Scopus subject areas

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