Syndecan-4 core protein is sufficient for the assembly of focal adhesions and actin stress fibers

Frank Echtermeyer, Peter C. Baciu, Stefania Saoncella, Yimin Ge, Paul F. Goetinck

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

The formation of focal adhesions and actin stress fibers on fibronectin is dependent on signaling through β1 integrins and the heparan sulfate proteoglycan syndecan-4, and we have analyzed the requirement of the glycosaminoglycan chains of syndecan-4 during these events. Chinese hamster ovary cells with mutations in key enzymes of the glycanation process do not synthesize glycosaminoglycan chains and are unable to assemble actin stress fibers and focal contacts when cultured on fibronectin. Transfection of the mutant cells with a cDNA that encodes the core protein of chicken syndecan-4 leads to the production of unglycanated core protein. The overexpression of syndecan-4 core protein in these mutant cells increases cell spreading and is sufficient for these cells to assemble actin stress fibers and focal adhesions similar to wild-type cells seeded on fibronectin and vitronectin matrices. Syndecan-4 core protein colocalizes to focal contacts in mutant cells that have been transfected with the syndecan-4 core protein cDNA. These data indicate an essential role for the core protein of syndecan-4 in the generation of signals leading to actin stress fiber and focal contact assembly.

Original languageEnglish (US)
Pages (from-to)3433-3441
Number of pages9
JournalJournal of Cell Science
Volume112
Issue number20
StatePublished - 1999

Keywords

  • Actin cytoskeleton
  • Cell attachment
  • Chinese hamster ovary cell
  • Heparan sulfate proteoglycan
  • Syndecan

ASJC Scopus subject areas

  • Cell Biology

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