Surgical techniques of arterialized orthotopic liver transplantation in rats

Yi Ma, Guo Dong Wang, Zhi Yong Guo, Zhi Gang Guo, Xiao Shun He, Gui Hua Chen

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Background: Recently, much attention has been paid to hepatic artery reconstruction in rat liver transplantation, which can prevent bile duct ischemia and preserve better liver structure. In this study, three methods of graft arterialization, including sleeve, cuff, and stent anastomosis, were conducted and the results were compared. Methods: Orthotopic liver transplantation (OLT) with rearterialization was conducted in 90 rats, which were divided into sleeve, cuff, and stent groups (n=30 in each). Ninety-six rats received OLTs with standardized two-cuff technique without rearterialization as a control. The sleeve technique included an end-to-end anastomosis between the donor common hepatic artery and recipient proper hepatic artery, or between the donor celiac artery and recipient common hepatic artery. Cuff technique involved an anastomosis between the donor common hepatic artery and recipient common hepatic artery. In the stent technique, the recipient hepatic artery and donor hepatic artery were connected using an intraluminal polyethylene stent. The arterial anastomosis time and arterial patency rate in each group were recorded. The liver graft survival and bile duct complication rates were measured. Results: The total surgical time of OLT with rearterialization was (118.3±12.9) minutes in the sleeve group, (106.2±11.6) minutes in the cuff, (93.8±10.2) minutes in the stent, and (88.2±9.6) minutes in the control. The corresponding anhepatic phase was (19.6±2.8), (19.2±2.2), (18.6±1.8), and (20.0±2.5) minutes respectively in the sleeve, cuff, stent, and control groups. One-week survival rate was 86.5% in the control, and 86.7% in the groups with rearterialization. No significant difference was detected in the survival rate between them (P>0.05). The incidence of biliary complications in non-rearterialized group (17.7%) was significantly higher than that in the rearterialized group (6.70%, P<0.05). No significant difference was found in the incidence of biliary complications among the three rearterialized groups (P>0.05). Conclusions: The OLT with rearterialization is more physiological than that without rearterialization, and leads to a lower rate of bile duct complications. Among the three methods of rearterialization, sleev anastomosis associated with a higher survival rate, allowing less dissection and less injury to the surrounding tissues.

Original languageEnglish (US)
Pages (from-to)1914-1917
Number of pages4
JournalChinese Medical Journal
Volume120
Issue number21
DOIs
StatePublished - Nov 5 2007

Keywords

  • Hepatic rearterialization
  • Liver transplantation
  • Rat

ASJC Scopus subject areas

  • Medicine(all)

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