1H and 13C NMR characterization and stereochemical assignments of bile acids in aqueous media

Omkar B. Ijare, B. S. Somashekar, Y. Jadegoud, G. A.Nagana Gowda

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

The unconjugated bile acids cholic acid, deoxycholic acid, and chenodeoxycholic acid; their glycine and taurine conjugates glycocholic acid, glycodeoxycholic acid, glycochenodeoxycholic acid, taurocholic acid, taurodeoxycholic acid, and taurochenodeoxycholic acid; and a taurine conjugated ursodeoxycholic acid, tauroursodeoxycholic acid, were characterized through 1H and 13C NMR in aqueous media under the physiological pH region (7.4 ± 0.1). Assignments of 1H and 13C signals of all the bile acids were made using a combination of several one- and two-dimensional, homonuclear (1H-1H) and heteronuclear (1H-13C) correlations as well as spectral editing NMR methods. Stereochemical assignment of the five-membered ring of the bile acids is reported here for the first time. The complete characterization of various bile acids in aqueous media presented here may have implications in the study of the pathophysiology of biliary diseases through human biliary fluids using NMR spectroscopy.

Original languageEnglish (US)
Pages (from-to)1031-1041
Number of pages11
JournalLipids
Volume40
Issue number10
DOIs
StatePublished - Oct 1 2005

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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