Suicide gene therapy toxicity after multiple and repeat injections in patients with localized prostate cancer

Moshe Shalev, Dov Kadmon, Bin S. Teh, Edward B. Butler, Estuardo Aguilar-Cordova, Timothy C. Thompson, James R. Herman, Howard L. Adler, Peter T. Scardino, Brian J. Miles

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

Purpose: We assess risks, toxicity and side effects of multiple and repeat in situ suicide gene therapy in patients with localized prostate cancer. Materials and Methods: The study population comprised patients with localized prostate cancer receiving multiple and/or repeat intraprostatic injections of a replication deficient adenovirus containing the herpes simplex virus thymidine kinase (HSV-tk) gene. Intravenous ganciclovir or oral valaciclovir was given for 14 days after injection. Patients were recruited from 4 different clinical protocols in studies of toxicity and efficacy of suicide gene therapy, and closely monitored for toxicity and side effects during and after treatment. Toxicity was graded according to the Cancer Therapy Evaluation Program common toxicity criteria published by the National Cancer Institute. Results: A total of 52 patients were treated under these clinical protocols with a total of 76 gene therapy cycles. Toxic events were recorded in 16 of 29 patients (55.2%) who were given multiple viral injections into the prostate, 7 of 20 (35%) who received 2 cycles of 'suicide' gene therapy and 3 of 4 (75%) who received a third course of gene therapy. All toxic events after multiple or repeat injections were mild (grades 1 to 2) and resolved completely once the therapy course was terminated. No additive toxicity was noted in patients receiving repeat gene therapy cycles. Mean followup was 12.8 months (range 3 to 34). Preliminary results for 28 patients in 2 clinical protocols indicated a mean decrease of 44% in PSA in 43%. Conclusions: Direct injection into the prostate of a replication defective adenovirus containing the HSV-tk gene followed by intravenous ganciclovir is safe even in repeat cycles.

Original languageEnglish (US)
Pages (from-to)1747-1750
Number of pages4
JournalJournal of Urology
Volume163
Issue number6
DOIs
StatePublished - Jun 2000

Keywords

  • Adenoviridae
  • Ganciclovir
  • Gene therapy
  • Prostate
  • Prostatic neoplasms

ASJC Scopus subject areas

  • Urology

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