Subarachnoid hemorrhage induces subacute and early chronic impairment in learning and memory in mice

Research output: Contribution to conferenceAbstractpeer-review


Background: Subarachnoid haemorrhage (SAH) leads to significant long-term cognitive deficits, the post-SAH syndrome.
Existing neurological scales used to assess outcomes of SAH are prevalently focused on sensorymotor functions.
Aim: To better evaluate short term and chronic consequences of SAH we explored and validated a battery of neurobehavioral tests to gauge the functional outcomes in mice after the circle of Willis perforation-induced SAH.
Method: Behaviour of Sham and SAH mice was compared using Garcia scale, open-field, Y-maze, dyadic social interaction, Barnes maze, and novel object recognition tests during 30 days postictal period.
Results/Conclusions: The 18-point Garcia scale, applied up to 4 days, detected impairment only at 24hrs with no significant differences between the groups. A decrease in locomotion in both groups was detected at 4-days postsurgery in the open field test but recovered at 30-days. However, an anxiety-like behaviour undetected at 4-days developed at 30-days in SAH mice. At 4-days post-surgery, Y-maze revealed an impairment in working spatial memory in SAH mice. Dyadic social interactions showed decreased sociability in SAH mice, which spent less time interacting with the stimulus mouse. At 30-days, SAH mice displayed spatial learning and memory deficits in the Barnes maze committing significantly more errors and used more time to find the escape box. Novel object recognition test revealed cognitive dysfunction in the SAH mice. Data suggest dysfunction of the limbic system and hippocampus in particular. This battery of 5 basic behavioural tests allows to detect neurocognitive deficits in a sub-acute and chronic phase following the SAH.
Original languageEnglish (US)
Number of pages71
StatePublished - Jun 1 2022
EventBrain '22 - UK, Glasgow, United Kingdom
Duration: May 28 2022Jun 1 2022


ConferenceBrain '22
Country/TerritoryUnited Kingdom


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