Studies on the Structure, Biosynthesis and Bacterial Metabolism of 15‐Hydroxylated Steroids in the Female Rat

Håkan Eriksson, Jan‐Åke Gustafsson, Jan SjÖVALL

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Gas‐liquid chromatography, gas chromatography‐mass spectrometry and radio‐gas chromatog‐raphy have been used to obtain a tentative identification of eight 15‐hydroxylated C21O4 steroids in faeces and urine from conventional female rats: 3α(and 3β),15α‐dihydroxy‐5α,14β‐pregnane‐11,20‐dione, 3α(and 3β),11β,15α‐trihydroxy‐5α,14β‐pregnan‐20‐one, 3α(and 3β),15α, 20β‐trihy‐droxy‐5α,14β‐pregnan‐11‐one and 5α,14β‐pregnan‐3α(and 3β),11β,15α,20β‐tetrol. The assign‐ment of stereochemistry is based on indirect evidence. The steroids were present in the free and monosulphate fractions and became labelled after administration of [4‐14C]pregnenolone and [4‐14C]corticosterone to the rats. Incubations in vitro with caecal contents from conventional rats showed that they were microbial metabolites of the 15α,21‐hydroxylated C21O5 steroids previously shown to be present in faeces and urine from germfree rats.

Original languageEnglish (US)
Pages (from-to)433-441
Number of pages9
JournalEuropean Journal of Biochemistry
Volume19
Issue number3
DOIs
StatePublished - Apr 1971

ASJC Scopus subject areas

  • Biochemistry

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