Studies on the Metabolism of C19‐Steroids in Rat Liver: 1. Hydroxylation of Testosterone in Rat Liver Microsomes

B. P. LISBOA; J. GUSTAFSSON; J. SJÖVALL

doi:10.1111/j.1432-1033.1968.tb00240.x

Studies on the Metabolism of C₁₉‐Steroids in Rat Liver: 1. Hydroxylation of Testosterone in Rat Liver Microsomes

B. P. LISBOA, J. GUSTAFSSON, J. SJÖVALL

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Abstract

The formation of polar C₁₉‐steroids with a 3‐oxo‐Δ⁴‐structure after incubation of testosterone with 105,000 ×g microsomes of adult male rat liver was investigated. The ultraviolet‐positive metabolites were isolated by thin‐layer chromatography. The isolated compounds were identified as 2β‐, 6β‐, 7α‐, and 16α‐hydroxytestosterone by means of gas‐liquid chromatography‐mass spectrometry. A dihydroxytesterone was also isolated and tentatively identified as 6β,16α,17β‐tri‐hydroxyandrost‐4‐en‐3‐one.

Original language	English (US)
Pages (from-to)	496-505
Number of pages	10
Journal	European Journal of Biochemistry
Volume	4
Issue number	4
DOIs	https://doi.org/10.1111/j.1432-1033.1968.tb00240.x
State	Published - May 1968

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Biochemistry

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10.1111/j.1432-1033.1968.tb00240.x

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title = "Studies on the Metabolism of C19‐Steroids in Rat Liver: 1. Hydroxylation of Testosterone in Rat Liver Microsomes",

abstract = "The formation of polar C19‐steroids with a 3‐oxo‐Δ4‐structure after incubation of testosterone with 105,000 ×g microsomes of adult male rat liver was investigated. The ultraviolet‐positive metabolites were isolated by thin‐layer chromatography. The isolated compounds were identified as 2β‐, 6β‐, 7α‐, and 16α‐hydroxytestosterone by means of gas‐liquid chromatography‐mass spectrometry. A dihydroxytesterone was also isolated and tentatively identified as 6β,16α,17β‐tri‐hydroxyandrost‐4‐en‐3‐one.",

author = "LISBOA, \{B. P.\} and J. GUSTAFSSON and J. SJ{\"O}VALL",

year = "1968",

month = may,

doi = "10.1111/j.1432-1033.1968.tb00240.x",

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journal = "European Journal of Biochemistry",

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AU - LISBOA, B. P.

AU - GUSTAFSSON, J.

AU - SJÖVALL, J.

PY - 1968/5

Y1 - 1968/5

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AB - The formation of polar C19‐steroids with a 3‐oxo‐Δ4‐structure after incubation of testosterone with 105,000 ×g microsomes of adult male rat liver was investigated. The ultraviolet‐positive metabolites were isolated by thin‐layer chromatography. The isolated compounds were identified as 2β‐, 6β‐, 7α‐, and 16α‐hydroxytestosterone by means of gas‐liquid chromatography‐mass spectrometry. A dihydroxytesterone was also isolated and tentatively identified as 6β,16α,17β‐tri‐hydroxyandrost‐4‐en‐3‐one.

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ER -

Studies on the Metabolism of C₁₉‐Steroids in Rat Liver: 1. Hydroxylation of Testosterone in Rat Liver Microsomes

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