Studies on the Metabolism of C19‐Steroids in Rat Liver: 1. Hydroxylation of Testosterone in Rat Liver Microsomes

B. P. LISBOA, Jan-Ake Gustafsson, J. SJÖVALL

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

The formation of polar C19‐steroids with a 3‐oxo‐Δ4‐structure after incubation of testosterone with 105,000 ×g microsomes of adult male rat liver was investigated. The ultraviolet‐positive metabolites were isolated by thin‐layer chromatography. The isolated compounds were identified as 2β‐, 6β‐, 7α‐, and 16α‐hydroxytestosterone by means of gas‐liquid chromatography‐mass spectrometry. A dihydroxytesterone was also isolated and tentatively identified as 6β,16α,17β‐tri‐hydroxyandrost‐4‐en‐3‐one.

Original languageEnglish (US)
Pages (from-to)496-505
Number of pages10
JournalEuropean Journal of Biochemistry
Volume4
Issue number4
DOIs
StatePublished - Jan 1 1968

ASJC Scopus subject areas

  • Biochemistry

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