After incubation of 4‐androstene‐3,17‐dione, 5α‐androstane‐3,17‐dione, 3α‐hydroxy‐5α‐androstan‐17‐one and 3β‐hydroxy‐5α‐androstan‐17‐one with rat liver microsomes the following steroids were isolated and identified by thin‐layer chromatography and by gas chromatography‐mass spectrometry: 3α‐ and 3β, 16α‐dihydroxy‐5α‐androstan‐17‐one, 3α‐ and 3β, 7α‐dihydroxy‐5α‐androstan‐17‐one, 3β, 7β‐dihydroxy‐5α‐androstan‐17‐one and 3β, 17β‐dihydroxy‐5α‐androstan‐16‐one. The importance of the 17‐oxo and 17β‐hydroxy pathways in the metabolism of C19 steroids in rat liver is discussed.
|Original language||English (US)|
|Number of pages||6|
|Journal||European Journal of Biochemistry|
|State||Published - Jan 1 1970|
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