TY - JOUR
T1 - Studies on the metabolism of C19 steroids in rat liver. 7. 18-hydroxylation of 17-oxo-C19 steroids in rat liver microsomes
AU - Gustafsson, J. Å
AU - Lisboa, B. P.
N1 - Funding Information:
We are sincerely grateful to Dr. D.K. Fukushima for providing us with the necessary reference steroids. The skilful technical assistance of Miss Gun Elisabeth Larsson is gratefully acknowledged. This investigation was supported by grants from the Swedish Medical Research Council to B.L. (No. 13X-2525) and to Dr. J. SjSvall (No. 13X-219). The authors are indebted to "Syskonen Wess4ns Stiftelse" for a fellowship awarded to one of us (J.-A.G.).
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1970/6
Y1 - 1970/6
N2 - After incubation of 4-androstene-3, 17-dione, 5α-androstane-3, 17-dione, 3α-hydroxy-5α-androstan-17-one, 3β-hydroxy-5α-androstan-17-dione, 17β-hydroxy-4-androsten-3-one, and 17β-hydroxy-5α-androstan-3-one with rat liver microsomes 18-hydroxy-4-androstene-3, 17-dione, 18-hydroxy-5α-androstane-3, 17-dione, 3α, 18-dihydroxy-5α-androstan-17-one, and 3β, 18-dihydroxy-5α-androstan-17-one have been isolated and identified by thin-layer chromatography and by gas chromatography-mass spectrometry. It is concluded that the liver may be an important extraadrenal site of 18-hydroxylation of steroids.
AB - After incubation of 4-androstene-3, 17-dione, 5α-androstane-3, 17-dione, 3α-hydroxy-5α-androstan-17-one, 3β-hydroxy-5α-androstan-17-dione, 17β-hydroxy-4-androsten-3-one, and 17β-hydroxy-5α-androstan-3-one with rat liver microsomes 18-hydroxy-4-androstene-3, 17-dione, 18-hydroxy-5α-androstane-3, 17-dione, 3α, 18-dihydroxy-5α-androstan-17-one, and 3β, 18-dihydroxy-5α-androstan-17-one have been isolated and identified by thin-layer chromatography and by gas chromatography-mass spectrometry. It is concluded that the liver may be an important extraadrenal site of 18-hydroxylation of steroids.
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U2 - 10.1016/S0039-128X(70)80042-3
DO - 10.1016/S0039-128X(70)80042-3
M3 - Article
C2 - 5429404
AN - SCOPUS:0014806398
SN - 0039-128X
VL - 15
SP - 723
EP - 735
JO - Steroids
JF - Steroids
IS - 6
ER -