Studies on the Metabolism of C19 Steroids in Rat Liver: 6α‐Hydroxylation of 3‐Oxo‐Δ4‐Steroids in Rat Liver Microsomes

Jan‐Åke Gustafsson; Belisário P. Lisboa

doi:10.1111/j.1432-1033.1970.tb00860.x

Studies on the Metabolism of C₁₉ Steroids in Rat Liver: 6α‐Hydroxylation of 3‐Oxo‐Δ⁴‐Steroids in Rat Liver Microsomes

Jan‐Åke Gustafsson, Belisário P. Lisboa

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Abstract

After incubation of testosterone and androstenedione with 105000μg microsomes of rat liver 6α‐hydroxytestosterone and 6α‐hydroxyandrostenedione, respectively, were isolated and identified by thin‐layer chromatography and gas chromatography‐mass spectrometry. This is the first example of 6α‐hydroxylation of steroid hormones in the rat and the findings are discussed in relation to earlier reports of 6α‐ and 6β‐hydroxylated steroids in mammalia.

Original language	English (US)
Pages (from-to)	369-374
Number of pages	6
Journal	European Journal of Biochemistry
Volume	12
Issue number	2
DOIs	https://doi.org/10.1111/j.1432-1033.1970.tb00860.x
State	Published - Feb 1970

ASJC Scopus subject areas

Biochemistry

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10.1111/j.1432-1033.1970.tb00860.x

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abstract = "After incubation of testosterone and androstenedione with 105000μg microsomes of rat liver 6α‐hydroxytestosterone and 6α‐hydroxyandrostenedione, respectively, were isolated and identified by thin‐layer chromatography and gas chromatography‐mass spectrometry. This is the first example of 6α‐hydroxylation of steroid hormones in the rat and the findings are discussed in relation to earlier reports of 6α‐ and 6β‐hydroxylated steroids in mammalia.",

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AU - Lisboa, Belisário P.

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N2 - After incubation of testosterone and androstenedione with 105000μg microsomes of rat liver 6α‐hydroxytestosterone and 6α‐hydroxyandrostenedione, respectively, were isolated and identified by thin‐layer chromatography and gas chromatography‐mass spectrometry. This is the first example of 6α‐hydroxylation of steroid hormones in the rat and the findings are discussed in relation to earlier reports of 6α‐ and 6β‐hydroxylated steroids in mammalia.

AB - After incubation of testosterone and androstenedione with 105000μg microsomes of rat liver 6α‐hydroxytestosterone and 6α‐hydroxyandrostenedione, respectively, were isolated and identified by thin‐layer chromatography and gas chromatography‐mass spectrometry. This is the first example of 6α‐hydroxylation of steroid hormones in the rat and the findings are discussed in relation to earlier reports of 6α‐ and 6β‐hydroxylated steroids in mammalia.

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Studies on the Metabolism of C₁₉ Steroids in Rat Liver: 6α‐Hydroxylation of 3‐Oxo‐Δ⁴‐Steroids in Rat Liver Microsomes

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