Studies on the metabolism of C19 steroids in rat liver. 5. 18-hydroxylation of 17β-hydroxy-C19 steroids in rat liver microsomes

J. Å Gustafsson; B. P. Lisboa

doi:10.1016/S0039-128X(69)80074-7

Studies on the metabolism of C₁₉ steroids in rat liver. 5. 18-hydroxylation of 17β-hydroxy-C₁₉ steroids in rat liver microsomes

J. Å Gustafsson, B. P. Lisboa

Houston Methodist

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

After incubation of testosterone, 18-hydroxytestosterone and 5α-dihydrotestosterone with rat liver microsomes 17β,18-dihydroxy-5α-androstan-3-one, 5α-androstane-3α,17β,18-triol and 5α-androstane-3β,17β,18-triol have been isolated and characterized by thin-layer and gas-liquid chromatography and by gas chromatography-mass spectrometry. Furthermore, after incubation of 5α-dihydrotestosterone two androstanetetrols were isolated, the mass spectrometric characteristics of which indicate the presence of an 18-hydroxyl group.

Original language	English (US)
Pages (from-to)	659-674
Number of pages	16
Journal	Steroids
Volume	14
Issue number	6
DOIs	https://doi.org/10.1016/S0039-128X(69)80074-7
State	Published - Dec 1969

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology
Pharmacology
Clinical Biochemistry
Organic Chemistry

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10.1016/S0039-128X(69)80074-7

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@article{12f1c2758e6743f18a53401f399fbc0a,

title = "Studies on the metabolism of C19 steroids in rat liver. 5. 18-hydroxylation of 17β-hydroxy-C19 steroids in rat liver microsomes",

abstract = "After incubation of testosterone, 18-hydroxytestosterone and 5α-dihydrotestosterone with rat liver microsomes 17β,18-dihydroxy-5α-androstan-3-one, 5α-androstane-3α,17β,18-triol and 5α-androstane-3β,17β,18-triol have been isolated and characterized by thin-layer and gas-liquid chromatography and by gas chromatography-mass spectrometry. Furthermore, after incubation of 5α-dihydrotestosterone two androstanetetrols were isolated, the mass spectrometric characteristics of which indicate the presence of an 18-hydroxyl group.",

author = "Gustafsson, \{J. {\AA}\} and Lisboa, \{B. P.\}",

note = "Funding Information: We are sincerely grateful to Dr. D.K. Fukushima for providing us with the necessary reference steroids. The skilful technical assistance of Miss Gun Elisabeth Larsson and Miss Dagmar Boden is gratefully acknowledged. This investigation was supported by grants from the Swedish Medical Research Council to B.L. (No. 13X-2525) and to Dr. J. Sj\textasciitilde{}vall (No. 13X-219). The authors are indebted to {"}Syskonen Wess\textasciitilde{}ns Stiftelse{"} for a fellowship awarded to one of us (J.-A. G.) Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "1969",

month = dec,

doi = "10.1016/S0039-128X(69)80074-7",

language = "English (US)",

volume = "14",

pages = "659--674",

journal = "Steroids",

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publisher = "Elsevier",

number = "6",

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T1 - Studies on the metabolism of C19 steroids in rat liver. 5. 18-hydroxylation of 17β-hydroxy-C19 steroids in rat liver microsomes

AU - Gustafsson, J. Å

AU - Lisboa, B. P.

N1 - Funding Information: We are sincerely grateful to Dr. D.K. Fukushima for providing us with the necessary reference steroids. The skilful technical assistance of Miss Gun Elisabeth Larsson and Miss Dagmar Boden is gratefully acknowledged. This investigation was supported by grants from the Swedish Medical Research Council to B.L. (No. 13X-2525) and to Dr. J. Sj~vall (No. 13X-219). The authors are indebted to "Syskonen Wess~ns Stiftelse" for a fellowship awarded to one of us (J.-A. G.) Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 1969/12

Y1 - 1969/12

N2 - After incubation of testosterone, 18-hydroxytestosterone and 5α-dihydrotestosterone with rat liver microsomes 17β,18-dihydroxy-5α-androstan-3-one, 5α-androstane-3α,17β,18-triol and 5α-androstane-3β,17β,18-triol have been isolated and characterized by thin-layer and gas-liquid chromatography and by gas chromatography-mass spectrometry. Furthermore, after incubation of 5α-dihydrotestosterone two androstanetetrols were isolated, the mass spectrometric characteristics of which indicate the presence of an 18-hydroxyl group.

AB - After incubation of testosterone, 18-hydroxytestosterone and 5α-dihydrotestosterone with rat liver microsomes 17β,18-dihydroxy-5α-androstan-3-one, 5α-androstane-3α,17β,18-triol and 5α-androstane-3β,17β,18-triol have been isolated and characterized by thin-layer and gas-liquid chromatography and by gas chromatography-mass spectrometry. Furthermore, after incubation of 5α-dihydrotestosterone two androstanetetrols were isolated, the mass spectrometric characteristics of which indicate the presence of an 18-hydroxyl group.

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SN - 0039-128X

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JO - Steroids

JF - Steroids

IS - 6

ER -

Studies on the metabolism of C₁₉ steroids in rat liver. 5. 18-hydroxylation of 17β-hydroxy-C₁₉ steroids in rat liver microsomes

Abstract

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