TY - JOUR
T1 - Studies on the Metabolism of C19 Steroids in Rat Liver
T2 - 2. Biosynthesis of Hydroxylated Derivatives of 17 β‐Hydroxy‐5α‐Androstan‐3‐One in Rat Liver Microsomes
AU - Gustafsson, J.
AU - Lisboa, B. P.
AU - Sjövall, J.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1968/8
Y1 - 1968/8
N2 - The formation of polar, saturated C19steroids with a 3‐keto‐5α‐configuration after incubation of 17β‐hydroxyandrost‐4‐en‐3‐one (testosterone) with 105,000 ×g microsomes of adult male rat liver was investigated. The metabolites were isolated by thin‐layer chromatography and were identified as 2β‐,6β‐(tentatively), 7α‐, and 16α‐hydroxy‐5α‐dihydrotestosterone by means of gas chromatography‐mass spectrometry. The same compounds were also isolated after incubation with 2β‐,6β‐,7α‐, and 16α‐hydroxytestosterone, respectively. Incubation with 5α‐dihydrotestosterone, however, resulted in the formation of 2β‐,7α‐, and 16α‐hydroxy‐5α‐dihydrotestosterone whereas no 6β‐hydroxy‐5α‐dihydrotestosterone could be found.
AB - The formation of polar, saturated C19steroids with a 3‐keto‐5α‐configuration after incubation of 17β‐hydroxyandrost‐4‐en‐3‐one (testosterone) with 105,000 ×g microsomes of adult male rat liver was investigated. The metabolites were isolated by thin‐layer chromatography and were identified as 2β‐,6β‐(tentatively), 7α‐, and 16α‐hydroxy‐5α‐dihydrotestosterone by means of gas chromatography‐mass spectrometry. The same compounds were also isolated after incubation with 2β‐,6β‐,7α‐, and 16α‐hydroxytestosterone, respectively. Incubation with 5α‐dihydrotestosterone, however, resulted in the formation of 2β‐,7α‐, and 16α‐hydroxy‐5α‐dihydrotestosterone whereas no 6β‐hydroxy‐5α‐dihydrotestosterone could be found.
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U2 - 10.1111/j.1432-1033.1968.tb00389.x
DO - 10.1111/j.1432-1033.1968.tb00389.x
M3 - Article
C2 - 5680362
AN - SCOPUS:0014315936
VL - 5
SP - 437
EP - 443
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
SN - 0014-2956
IS - 3
ER -