Studies of the mechanism of spontaneous germline ecotropic provirus acquisition in mice.

L. F. Lock, E. Keshet, D. J. Gilbert, N. A. Jenkins, N. G. Copeland

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

SWR/J--RF/J hybrid mice spontaneously acquire new germline ecotropic proviruses at high frequency. We have performed ovarian transplantation and in situ hybridization studies to delineate the mechanism and developmental stage of germline provirus acquisition. In addition, we have developed a novel, efficient and simple method to introduce single copy proviruses into the mouse germline. The results reported here have direct implications for understanding how proviruses are acquired in the germline, for using murine leukemia viruses as insertional mutagens, and for using retroviral vectors to introduce foreign genes into the mouse germline.

Original languageEnglish (US)
Pages (from-to)4169-4177
Number of pages9
JournalThe EMBO journal
Volume7
Issue number13
DOIs
StatePublished - Dec 20 1988

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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