Studies of the mechanism by which gangliosides inhibit the proliferative response of murine splenocytes to concanavalin A

Donald M. Marcus, Alicia Dustira, Isabel Diego, Susan Osovitz, Dorothy E. Lewis

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Gangliosides are known to inhibit the proliferative response of murine and human lymphocytes to antigens and mitogens in vitro. In this study the response of murine spleen cells to concanavalin A (Con A) was used as a model system. Analysis of the cellular events by flow cytometry revealed that during the first 24 hr of culture the effect of gangliosides on Con A-treated cells was minimal. At 48 hr, however, more of the ganglioside-treated cells were in G0 G1, the cells contained more RNA, and fewer cells were in S phase. These data indicate that gangliosides inhibit the transition of the cells from G0 G1 into the S phase of the cell cycle. Expression of the interleukin 2 (IL-2) receptor, as measured by the binding of a monoclonal antibody to the receptor, was not inhibited by the gangliosides. Binding of 125I-labeled recombinant IL-2 to cells cultured for 48 hr with Con A was inhibited by ganglioside GDla but not by asialo GMI. Inhibition was much more effective if the gangliosides were preincubated with IL-2 before addition of cells, but no inhibition was observed if the cells were preincubated with gangliosides and the unbound gangliosides were washed out prior to addition of the IL-2. These data suggest that interference with the binding of IL-2 to the high-affinity IL-2 receptor of activated T lymphocytes plays an important role in the inhibition of Con A-induced proliferation.

Original languageEnglish (US)
Pages (from-to)71-78
Number of pages8
JournalCellular Immunology
Volume104
Issue number1
DOIs
StatePublished - Jan 1987

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Studies of the mechanism by which gangliosides inhibit the proliferative response of murine splenocytes to concanavalin A'. Together they form a unique fingerprint.

Cite this