Structure of nascent replicative form DNA of coliphage M13

S. Dasgupta, S. Mitra

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Nascent replicative form type II (RFII) DNA of coliphage M13 synthesized in an Escherichia coli mutant deficient in the 5'→3' exonuclease associated with DNA polymerase I contains ribonucleotides that are retained in the covalently closed RFI DNA sealed in vitro by the joint action of T5 phage DNA polymerase and T4 phage DNA ligase. These RFI molecules are labile to alkali and RNase H, unlike the RFI produced either in vivo or from RFII with E. coli DNA polymerase I and E. coli DNA ligase. The ribonucleotides are located at one site and predominantly in one strand of the nascent RF DNA. Furthermore, these molecules contain multiple small gaps, randomly located, and one large gap in the intracistronic region.

Original languageEnglish (US)
Pages (from-to)153-157
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume75
Issue number1
DOIs
StatePublished - 1978

ASJC Scopus subject areas

  • General

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