TY - JOUR
T1 - Structure-function and pathogenesis studies of streptococcus pyogenes extracellular cysteine protease
AU - Burns, Eugene H.
AU - Marciel, Ann Marie
AU - Musser, James M.
PY - 1997
Y1 - 1997
N2 - Replacement of the single cysteine residue (C192) with serine in the Streptococcus pyogenes extracellular cysteine protease (SCP) prevented auto- catalytic processing of the 40-kDa zymogen to the 28-kDa mature form and eliminated proteolytic activity. SCP incubated with human endothelial cells induced a time- and concentration-dependent increase in a 66-kDa gelatinase/type IV collagenase in culture supernatants. Activation of this gelatinase/collagenase may contribute to endothelial cell damage, tissue destruction, and hemodynamic derangement observed in some patients with severe, invasive S. pyogenes infection.
AB - Replacement of the single cysteine residue (C192) with serine in the Streptococcus pyogenes extracellular cysteine protease (SCP) prevented auto- catalytic processing of the 40-kDa zymogen to the 28-kDa mature form and eliminated proteolytic activity. SCP incubated with human endothelial cells induced a time- and concentration-dependent increase in a 66-kDa gelatinase/type IV collagenase in culture supernatants. Activation of this gelatinase/collagenase may contribute to endothelial cell damage, tissue destruction, and hemodynamic derangement observed in some patients with severe, invasive S. pyogenes infection.
UR - http://www.scopus.com/inward/record.url?scp=0030770564&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030770564&partnerID=8YFLogxK
U2 - 10.1007/978-1-4899-1825-3_136
DO - 10.1007/978-1-4899-1825-3_136
M3 - Article
C2 - 9331720
AN - SCOPUS:0030770564
SN - 0065-2598
VL - 418
SP - 589
EP - 592
JO - Advances in experimental medicine and biology
JF - Advances in experimental medicine and biology
ER -