Structure, expression, and chromosome mapping of LATS2, a mammalian homologue of the Drosophila tumor suppressor gene lats/warts

Norikazu Yabuta, Takayuki Fujii, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Hiroaki Nishiguchi, Yuichi Endo, Shingo Toji, Hiromitsu Tanaka, Yoshitake Nishimune, Hiroshi Nojima

Research output: Contribution to journalArticlepeer-review

91 Scopus citations

Abstract

We have cloned and characterized LATS2, a novel mammalian homologue of the Drosophila tumor suppressor gene lats/warts. Northern blot analysis showed ubiquitous expression of mouse LATS2 (MmLATS2) mRNA, whereas expression of human LATS2 (HsLATS2) mRNA was enhanced in skeletal muscle and heart. Immunoblotting analysis of fractionated cell lysates showed HsLats2 to be a nuclear protein. We mapped the MmLATS2 gene to mouse chromosome 14 by interspecific backcross analysis. We also mapped the HsLATS2 gene (by fluorescence in situ hybridization) to the 13q11-q12 region, in which a loss of heterozygosity has been frequently observed in many primary cancers and to which the tumor suppressor genes RB and BRCA2 have also been mapped. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)263-270
Number of pages8
JournalGenomics
Volume63
Issue number2
DOIs
StatePublished - Jan 15 2000

ASJC Scopus subject areas

  • Genetics

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