TY - JOUR
T1 - Structure, chromosomal localization, and expression analysis of the mouse inhibin/activin βc (Inhbc) gene
AU - Schmitt, Jacky
AU - Hötten, Gertrud
AU - Jenkins, Nancy A.
AU - Gilbert, Debra J.
AU - Copeland, Neal G.
AU - Pohl, Jens
AU - Schrewe, Heinrich
N1 - Funding Information:
We thank Drs. GuÈnther SchuÈtz and Wolfgang Schmid for providing us the mouse liver cDNA library. We acknowledge Renate Mielke and Debbie Barnhart for excellent technical assistance. We are grateful to our colleagues Drs. Ernst-Martin FuÈchtbauer, Matthias He-brok, John McLaughlin, Davor Solter, and Grace Wei for critical reading and comments about the manuscript. This research was supported, in part, by the American National Cancer Institute, DHHS, under a contract with ABL. J.S. was the recipient of a Max-Planck-Society postdoctoral fellowship.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1996/3/15
Y1 - 1996/3/15
N2 - The mouse inhibin/activin βc gene (Inhbc), a member of the transforming growth factor-β (TGF-β) superfamily, was cloned, mapped, and characterized. The gene spans approximately 14 kb, is composed of two exons, and maps to the distal region of mouse chromosome 10, which is syntenic to chromosome 12q13.1, where the human inhibin/activin βc gene (INHBC) maps. The primary translation product is a preproprotein of 352 amino acids. The mature C-terminal domain of 116 amino acids shares 94% identity with its human homolog. Primer extension analysis shows that transcription starts approximately 130 bp upstream of the translation initiation site, and no TATA box was found in the promoter. Ribonuclease protection analyses reveal that mouse Inhbc is predominantly expressed in adult liver. Embryonic expression is detected beginning from Day 14.5 of gestation.
AB - The mouse inhibin/activin βc gene (Inhbc), a member of the transforming growth factor-β (TGF-β) superfamily, was cloned, mapped, and characterized. The gene spans approximately 14 kb, is composed of two exons, and maps to the distal region of mouse chromosome 10, which is syntenic to chromosome 12q13.1, where the human inhibin/activin βc gene (INHBC) maps. The primary translation product is a preproprotein of 352 amino acids. The mature C-terminal domain of 116 amino acids shares 94% identity with its human homolog. Primer extension analysis shows that transcription starts approximately 130 bp upstream of the translation initiation site, and no TATA box was found in the promoter. Ribonuclease protection analyses reveal that mouse Inhbc is predominantly expressed in adult liver. Embryonic expression is detected beginning from Day 14.5 of gestation.
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U2 - 10.1006/geno.1996.0130
DO - 10.1006/geno.1996.0130
M3 - Article
C2 - 8838799
AN - SCOPUS:0029965175
VL - 32
SP - 358
EP - 366
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 3
ER -