Structure, chromosomal localization, and expression analysis of the mouse inhibin/activin βc (Inhbc) gene

Jacky Schmitt, Gertrud Hötten, Nancy A. Jenkins, Debra J. Gilbert, Neal G. Copeland, Jens Pohl, Heinrich Schrewe

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

The mouse inhibin/activin βc gene (Inhbc), a member of the transforming growth factor-β (TGF-β) superfamily, was cloned, mapped, and characterized. The gene spans approximately 14 kb, is composed of two exons, and maps to the distal region of mouse chromosome 10, which is syntenic to chromosome 12q13.1, where the human inhibin/activin βc gene (INHBC) maps. The primary translation product is a preproprotein of 352 amino acids. The mature C-terminal domain of 116 amino acids shares 94% identity with its human homolog. Primer extension analysis shows that transcription starts approximately 130 bp upstream of the translation initiation site, and no TATA box was found in the promoter. Ribonuclease protection analyses reveal that mouse Inhbc is predominantly expressed in adult liver. Embryonic expression is detected beginning from Day 14.5 of gestation.

Original languageEnglish (US)
Pages (from-to)358-366
Number of pages9
JournalGenomics
Volume32
Issue number3
DOIs
StatePublished - Mar 15 1996

ASJC Scopus subject areas

  • Genetics

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