TY - JOUR
T1 - Structure and mapping of the G protein γ3 subunit gene and a divergently transcribed novel gene, Gng3lg
AU - Downes, Gerald B.
AU - Copeland, Neal G.
AU - Jenkins, Nancy A.
AU - Gautam, N.
N1 - Funding Information:
Nucleotide sequence data from this article have been deposited with the GenBank Data Library under Accession Nos. AF069953, AF069954, and AF069955. This work was supported by National Institutes of Health Grant GM 46963 and the National Cancer Institute, DHHS, under contract with ABL. 1Supported by NIGMS predoctoral fellowship GM 17289-04. 2To whom correspondence should be addressed. Telephone: (314) 362-8568. Fax: (314) 362-8571. E-mail: [email protected].
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1998/10/15
Y1 - 1998/10/15
N2 - The mammalian nervous system is rich in signaling mediated by heterotrimeric (αβγ) G proteins. As an initial step to define the roles that particular γ subunit types play in signaling, we have begun to clone and characterize those genes that encode γ subunits enriched within neural tissue. In the present study, we have isolated and characterized the mouse γ3 subunit gene (Gng3). The γ3 subunit is expressed abundantly in the brain and at low levels in testes. Gng3 is composed of three exons spanning ~1.4 kb. A comparison of Gng3 with the gene structure for five other γ subtypes indicates that although these proteins are diverse at the amino acid level, their exon-intron boundaries are conserved. Sequence analysis of the 5' flanking region of Gng3 revealed the presence of a novel gene, the γ3 linked gene (Gng3lg). Gng3 and Gng3lg are organized in a head-to-head fashion with major transcription initiation sites separated by approximately 133 bp. Sequence analysis of a Gng3lg cDNA clone revealed an open reading frame encoding a 410-amino-acid protein of unknown function. Gng3lg transcripts are expressed in a variety of tissues including both brain and testes. Using an interspecific backcross panel, we localized both Gng3 and Gng3lg to the same locus on chromosome 19. The orientation, close proximity, and expression pattern of these two genes raise the distinct possibility that shared regulatory elements are used to control their expression.
AB - The mammalian nervous system is rich in signaling mediated by heterotrimeric (αβγ) G proteins. As an initial step to define the roles that particular γ subunit types play in signaling, we have begun to clone and characterize those genes that encode γ subunits enriched within neural tissue. In the present study, we have isolated and characterized the mouse γ3 subunit gene (Gng3). The γ3 subunit is expressed abundantly in the brain and at low levels in testes. Gng3 is composed of three exons spanning ~1.4 kb. A comparison of Gng3 with the gene structure for five other γ subtypes indicates that although these proteins are diverse at the amino acid level, their exon-intron boundaries are conserved. Sequence analysis of the 5' flanking region of Gng3 revealed the presence of a novel gene, the γ3 linked gene (Gng3lg). Gng3 and Gng3lg are organized in a head-to-head fashion with major transcription initiation sites separated by approximately 133 bp. Sequence analysis of a Gng3lg cDNA clone revealed an open reading frame encoding a 410-amino-acid protein of unknown function. Gng3lg transcripts are expressed in a variety of tissues including both brain and testes. Using an interspecific backcross panel, we localized both Gng3 and Gng3lg to the same locus on chromosome 19. The orientation, close proximity, and expression pattern of these two genes raise the distinct possibility that shared regulatory elements are used to control their expression.
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U2 - 10.1006/geno.1998.5508
DO - 10.1006/geno.1998.5508
M3 - Article
C2 - 9790771
AN - SCOPUS:17144462206
SN - 0888-7543
VL - 53
SP - 220
EP - 230
JO - Genomics
JF - Genomics
IS - 2
ER -