Structural insights into the mode of action of a pure antiestrogen

Ashley C.W. Pike, A. Marek Brzozowski, Julia Walton, Roderick E. Hubbard, Ann Gerd Thorsell, Yi Lin Li, Jan Åke Gustafsson, Mats Carlquist

Research output: Contribution to journalArticlepeer-review

310 Scopus citations

Abstract

Background: Estrogens exert their effects on target tissues by binding to a nuclear transcription factor termed the estrogen receptor (ER). Previous structural studies have demonstrated that each class of ER ligand (agonist, partial agonist, and SERM antagonist) induces distinctive orientations in the receptor's carboxy-terminal transactivation helix. The conformation of this portion of the receptor determines whether ER can recruit and interact with the components of the transcriptional machinery, thereby facilitating target gene expression. Results: We have determined the structure of rat ERβ ligand binding domain (LBD) in complex with the pure antiestrogen ICI 164,384 at 2.3 Å resolution. The binding of this compound to the receptor completely abolishes the association between the transactivation helix (H12) and the rest of the LBD. The structure reveals that the terminal portion of ICI's bulky side chain substituent protrudes from the hormone binding pocket, binds along the coactivator recruitment site, and physically prevents H12 from adopting either its characteristic agonist or AF2 antagonist orientation. Conclusions: The binding mode adopted by the pure antiestrogen is similar to that seen for other ER antagonists. However, the size and resultant positioning of the ligand's side chain substituent produces a receptor conformation that is distinct from that adopted in the presence of other classes of ER ligands. The novel observation that binding of ICI results in the complete destabilization of H12 provides some indications as to a possible mechanism for pure receptor antagonism.

Original languageEnglish (US)
Pages (from-to)145-153
Number of pages9
JournalStructure
Volume9
Issue number2
DOIs
StatePublished - 2001

Keywords

  • Estrogen receptor
  • ICI 164,384
  • Pure antiestrogen
  • Receptor antagonism
  • Transactivation

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

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