Structural evidence of anti-atherogenic microRNAs

Anthony Virtue, Jietang Mai, Ying Yin, Shu Meng, Tran Tran, Xiaohua Jiang, Hong Wang, Xiao Feng Yang

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Our research attempted to address two important questions - how microRNAs modulate atherogenic inflammatory genes from a panoramic viewpoint and whether their augmented expression results from reduced microRNAs suppression. To resolve these knowledge gaps, we employed a novel database mining technique in conjunction with statistical analysis criteria established from experimentally verified microRNAs. We found that the expression of 33 inflammatory genes up-regulated in atherosclerotic lesions contain structural features in the 3'UTR of their mRNAs for potential microRNAs regulation. Additionally, the binding features governing the interactions between the microRNAs and the inflammatory gene mRNA were statistically identical to the features of experimentally verified microRNAs. Furthermore, 21 of the 33 inflammatory genes (64%) were targeted by highly expressed microRNAs and 10 of these (48%) were targeted by a single microRNA, suggesting microRNA regulation specificity. Supplementing our findings, 7 out of the 20 unique microRNAs (35%) were previously confirmed to be down-regulated when treated with proatherogenic factors. These results indicate a critical role of anti-inflammatory microRNAs in suppressing pro-atherogenic inflammatory gene expression.

Original languageEnglish (US)
Pages (from-to)3133-3145
Number of pages13
JournalFrontiers in Bioscience
Volume16
Issue number7
DOIs
StatePublished - Jun 1 2011

Keywords

  • Atherosclerosis
  • Inflammatory genes
  • MicroRNAs
  • Vascular inflammation
  • mRNA stability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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