TY - JOUR
T1 - Structural comparison and chromosomal localization of the human and mouse IL-13 genes
AU - McKenzie, A. N.J.
AU - Li, X.
AU - Largaespada, D. A.
AU - Sato, A.
AU - Kaneda, A.
AU - Zurawski, S. M.
AU - Doyle, E. L.
AU - Milatovich, A.
AU - Francke, U.
AU - Copeland, Neal G.
AU - Jenkins, Nancy A.
AU - Zurawski, G.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The genomic structure of the recently described cytokine IL-13 has been determined for both human and mouse genes. The nucleotide sequence of a 4.6-kb DNA segment of the human gene is described. The human IL-13 gene (IL13) occurs as a single copy in the haploid genome and maps to human chromosome 5. A 4.3-kb DNA fragment of the mouse IL-13 gene (Il13) has been sequenced and found to occur as a single copy, mapping to mouse chromosome 11. Intrachromosomal mapping studies revealed that both genes contain four exons and three introns and show a high degree of sequence identity throughout their length. Potential recognition sequences for transcription factors that are present in the 5†-flanking region and are conserved between both genes include IFN-responsive elements, binding sites for AP-1, AP-2 and AP-3, an NF-IL 6 site, and a TATA-like sequence. Both genes map to chromosomal locations adjacent to genes encoding other cytokines, including IL-3, GM-CSF, IL-5, and IL-4, suggesting that IL-13 is another member of this cytokine gene family that may have arisen by gene duplication.
AB - The genomic structure of the recently described cytokine IL-13 has been determined for both human and mouse genes. The nucleotide sequence of a 4.6-kb DNA segment of the human gene is described. The human IL-13 gene (IL13) occurs as a single copy in the haploid genome and maps to human chromosome 5. A 4.3-kb DNA fragment of the mouse IL-13 gene (Il13) has been sequenced and found to occur as a single copy, mapping to mouse chromosome 11. Intrachromosomal mapping studies revealed that both genes contain four exons and three introns and show a high degree of sequence identity throughout their length. Potential recognition sequences for transcription factors that are present in the 5†-flanking region and are conserved between both genes include IFN-responsive elements, binding sites for AP-1, AP-2 and AP-3, an NF-IL 6 site, and a TATA-like sequence. Both genes map to chromosomal locations adjacent to genes encoding other cytokines, including IL-3, GM-CSF, IL-5, and IL-4, suggesting that IL-13 is another member of this cytokine gene family that may have arisen by gene duplication.
UR - http://www.scopus.com/inward/record.url?scp=0027289274&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027289274&partnerID=8YFLogxK
M3 - Article
C2 - 8099936
AN - SCOPUS:0027289274
VL - 150
SP - 5436
EP - 5444
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 12
ER -