TY - JOUR
T1 - Streptococcus pyogenes causing toxic-shock-like syndrome and other invasive diseases
T2 - Clonal diversity and pyrogenic exotoxin expression
AU - Musser, James M.
AU - Hauser, Alan R.
AU - Kim, Michael H.
AU - Schlievert, Patrick M.
AU - Nelson, Kimberlyn
AU - Selander, Robert K.
PY - 1991/4/1
Y1 - 1991/4/1
N2 - Genetic diversity and relationships among 108 isolates of the bacterium Streptococcus pyogenes recently recovered from patients in the United States with toxic-shock-like syndrome or other invasive diseases were estimated by multilocus enzyme electrophoresis. Thirty-three electrophoretic types (ETs), representing distinctive multilocus clonal genotypes, were identified, but nearly half the disease episodes, including more than two-thirds of the cases of toxic-shock-like syndrome, were caused by strains of two related clones (ET 1 and ET 2). These two clones were also represented by recent pathogenic European isolates. A previous report of a relatively high frequency of expression of exotoxin A among isolates recovered from toxic-shock-like syndrome patients in the United States was confirmed; and the demonstration of this association both within clones and among distantly related clones supports the hypothesis that exotoxin A is a causal factor in pathogenesis of this disease. Near identity of the nucleotide sequences of the exotoxin A structural gene of six isolates of five ETs in diverse phylogenetic lineages was interpreted as evi-dence that the gene has been horizontally distributed among clones, presumably by bacteriophage-mediated transfer.
AB - Genetic diversity and relationships among 108 isolates of the bacterium Streptococcus pyogenes recently recovered from patients in the United States with toxic-shock-like syndrome or other invasive diseases were estimated by multilocus enzyme electrophoresis. Thirty-three electrophoretic types (ETs), representing distinctive multilocus clonal genotypes, were identified, but nearly half the disease episodes, including more than two-thirds of the cases of toxic-shock-like syndrome, were caused by strains of two related clones (ET 1 and ET 2). These two clones were also represented by recent pathogenic European isolates. A previous report of a relatively high frequency of expression of exotoxin A among isolates recovered from toxic-shock-like syndrome patients in the United States was confirmed; and the demonstration of this association both within clones and among distantly related clones supports the hypothesis that exotoxin A is a causal factor in pathogenesis of this disease. Near identity of the nucleotide sequences of the exotoxin A structural gene of six isolates of five ETs in diverse phylogenetic lineages was interpreted as evi-dence that the gene has been horizontally distributed among clones, presumably by bacteriophage-mediated transfer.
KW - Clones
KW - DNA sequencing
KW - Horizontal gene transfer
KW - Multilocus enzyme genotypes
KW - PCR
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U2 - 10.1073/pnas.88.7.2668
DO - 10.1073/pnas.88.7.2668
M3 - Article
C2 - 1672766
AN - SCOPUS:0025790953
VL - 88
SP - 2668
EP - 2672
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 7
ER -