Steroids in newborns and infants identification of steroids in urine from newborn infants

C. H.L. Shackleton, Jan-Ake Gustafsson, J. Sjövall

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

The steroids present in urine from newborn infants (1-6 days old) have been studied. The steroid conjugates were extracted from the urine by Amberlite XAD-2 resin and were hydrolysed by enzymes. Following extraction the steroids were analysed by combined gas chromatography-mass spectrometry. Of the steroids identified 3α,6α-dihydroxy-5β-pregnan-20-one, 3α,16α-dihydroxy-5β-pregnan-20-one, 5ξ-pregnane-3ξ,16ξ,20ξ-triol, 3β-hydroxy-5,16-pregnadien-20-one, 5-pregnene-3β,20α,21-triol, 5-pregnene-3β,16α,20α,21-tetrol, 3β,16α,21-trihydroxy-5-pregnen-20-one and 5-pregnene-3β,16ξ,17α,20ξ-tetrol have not previously been reported as occurring in urine from infants. The approximate rate of excretion (μg/24 h) of these steroids is reported.

Original languageEnglish (US)
Pages (from-to)265-280
Number of pages16
JournalSteroids
Volume17
Issue number1-5
DOIs
StatePublished - Jan 1 1971

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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