Steroids in newborns and infants identification of steroids in urine from newborn infants

C. H.L. Shackleton; Jan-Ake Gustafsson; J. Sjövall

doi:10.1016/S0039-128X(71)80129-0

Steroids in newborns and infants identification of steroids in urine from newborn infants

C. H.L. Shackleton, Jan-Ake Gustafsson, J. Sjövall

Houston Methodist

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

The steroids present in urine from newborn infants (1-6 days old) have been studied. The steroid conjugates were extracted from the urine by Amberlite XAD-2 resin and were hydrolysed by enzymes. Following extraction the steroids were analysed by combined gas chromatography-mass spectrometry. Of the steroids identified 3α,6α-dihydroxy-5β-pregnan-20-one, 3α,16α-dihydroxy-5β-pregnan-20-one, 5ξ-pregnane-3ξ,16ξ,20ξ-triol, 3β-hydroxy-5,16-pregnadien-20-one, 5-pregnene-3β,20α,21-triol, 5-pregnene-3β,16α,20α,21-tetrol, 3β,16α,21-trihydroxy-5-pregnen-20-one and 5-pregnene-3β,16ξ,17α,20ξ-tetrol have not previously been reported as occurring in urine from infants. The approximate rate of excretion (μg/24 h) of these steroids is reported.

Original language	English (US)
Pages (from-to)	265-280
Number of pages	16
Journal	Steroids
Volume	17
Issue number	1-5
DOIs	https://doi.org/10.1016/S0039-128X(71)80129-0
State	Published - Jan 1 1971

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology
Pharmacology
Clinical Biochemistry
Organic Chemistry

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10.1016/S0039-128X(71)80129-0

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title = "Steroids in newborns and infants identification of steroids in urine from newborn infants",

abstract = "The steroids present in urine from newborn infants (1-6 days old) have been studied. The steroid conjugates were extracted from the urine by Amberlite XAD-2 resin and were hydrolysed by enzymes. Following extraction the steroids were analysed by combined gas chromatography-mass spectrometry. Of the steroids identified 3α,6α-dihydroxy-5β-pregnan-20-one, 3α,16α-dihydroxy-5β-pregnan-20-one, 5ξ-pregnane-3ξ,16ξ,20ξ-triol, 3β-hydroxy-5,16-pregnadien-20-one, 5-pregnene-3β,20α,21-triol, 5-pregnene-3β,16α,20α,21-tetrol, 3β,16α,21-trihydroxy-5-pregnen-20-one and 5-pregnene-3β,16ξ,17α,20ξ-tetrol have not previously been reported as occurring in urine from infants. The approximate rate of excretion (μg/24 h) of these steroids is reported.",

author = "Shackleton, {C. H.L.} and Jan-Ake Gustafsson and J. Sj{\"o}vall",

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doi = "10.1016/S0039-128X(71)80129-0",

language = "English (US)",

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AU - Shackleton, C. H.L.

AU - Gustafsson, Jan-Ake

AU - Sjövall, J.

PY - 1971/1/1

Y1 - 1971/1/1

N2 - The steroids present in urine from newborn infants (1-6 days old) have been studied. The steroid conjugates were extracted from the urine by Amberlite XAD-2 resin and were hydrolysed by enzymes. Following extraction the steroids were analysed by combined gas chromatography-mass spectrometry. Of the steroids identified 3α,6α-dihydroxy-5β-pregnan-20-one, 3α,16α-dihydroxy-5β-pregnan-20-one, 5ξ-pregnane-3ξ,16ξ,20ξ-triol, 3β-hydroxy-5,16-pregnadien-20-one, 5-pregnene-3β,20α,21-triol, 5-pregnene-3β,16α,20α,21-tetrol, 3β,16α,21-trihydroxy-5-pregnen-20-one and 5-pregnene-3β,16ξ,17α,20ξ-tetrol have not previously been reported as occurring in urine from infants. The approximate rate of excretion (μg/24 h) of these steroids is reported.

AB - The steroids present in urine from newborn infants (1-6 days old) have been studied. The steroid conjugates were extracted from the urine by Amberlite XAD-2 resin and were hydrolysed by enzymes. Following extraction the steroids were analysed by combined gas chromatography-mass spectrometry. Of the steroids identified 3α,6α-dihydroxy-5β-pregnan-20-one, 3α,16α-dihydroxy-5β-pregnan-20-one, 5ξ-pregnane-3ξ,16ξ,20ξ-triol, 3β-hydroxy-5,16-pregnadien-20-one, 5-pregnene-3β,20α,21-triol, 5-pregnene-3β,16α,20α,21-tetrol, 3β,16α,21-trihydroxy-5-pregnen-20-one and 5-pregnene-3β,16ξ,17α,20ξ-tetrol have not previously been reported as occurring in urine from infants. The approximate rate of excretion (μg/24 h) of these steroids is reported.

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ER -

Steroids in newborns and infants identification of steroids in urine from newborn infants

Abstract

ASJC Scopus subject areas

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