TY - JOUR
T1 - Steroids in Newborns and Infants
T2 - Identification of 24,25‐Dihydro‐Δ9(11)‐Lanosterol and 4α, 14α‐Dimethyl Substituted Sterols among the Esterified and Free Sterols in Human Meconium
AU - Eneroth, Peter
AU - Gustafsson, Jan‐Åke
AU - Nyström, Ernst
PY - 1969/12
Y1 - 1969/12
N2 - Lipid extracts of human meconium were separated by chromatography on silicic acid and lipophilic Sephadex. Trimethylsilyl ethers of sterols in the free and ester fractions were analyzed by gas chromatography‐mass spectrometry. Cholesterol and lathosterol made up 93% of the total sterols and were the predominating sterols in the unesterified fraction (relative abundance: 96%). The remaining 4% in this fraction consisted of lanosterol, 24,25‐dihydro‐Δ8‐lanosterol, 24,25‐dihydro‐Δ9‐lanosterol, 4α,14α‐dimethyl‐5α‐cholest‐7 (and 8)‐en‐3β‐ol, and Δ7‐ and Δ8‐methostenol. The sterol ester fraction contained 4,4‐dimethyl‐5α‐cholest‐8‐en‐3β‐ol, 4,4‐dimethyl‐5α‐cholest‐8,14‐dien‐3β‐ol, β‐sitostanol, cholesterol, lathoesterol, lanosterol, 24,25‐dihydro‐Δ8‐lanosterol, 4α,14α‐dimethyl‐5α‐cholest‐7(and 8)‐en‐3β, and Δ7‐ and Δ8‐methostenol. However, this fraction contained a smaller proportion of Δ7‐sterols than the free fraction. The percentage of cholesterol and lathosterol was also relatively smaller (75%) than in the free sterol fraction.
AB - Lipid extracts of human meconium were separated by chromatography on silicic acid and lipophilic Sephadex. Trimethylsilyl ethers of sterols in the free and ester fractions were analyzed by gas chromatography‐mass spectrometry. Cholesterol and lathosterol made up 93% of the total sterols and were the predominating sterols in the unesterified fraction (relative abundance: 96%). The remaining 4% in this fraction consisted of lanosterol, 24,25‐dihydro‐Δ8‐lanosterol, 24,25‐dihydro‐Δ9‐lanosterol, 4α,14α‐dimethyl‐5α‐cholest‐7 (and 8)‐en‐3β‐ol, and Δ7‐ and Δ8‐methostenol. The sterol ester fraction contained 4,4‐dimethyl‐5α‐cholest‐8‐en‐3β‐ol, 4,4‐dimethyl‐5α‐cholest‐8,14‐dien‐3β‐ol, β‐sitostanol, cholesterol, lathoesterol, lanosterol, 24,25‐dihydro‐Δ8‐lanosterol, 4α,14α‐dimethyl‐5α‐cholest‐7(and 8)‐en‐3β, and Δ7‐ and Δ8‐methostenol. However, this fraction contained a smaller proportion of Δ7‐sterols than the free fraction. The percentage of cholesterol and lathosterol was also relatively smaller (75%) than in the free sterol fraction.
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U2 - 10.1111/j.1432-1033.1969.tb00795.x
DO - 10.1111/j.1432-1033.1969.tb00795.x
M3 - Article
C2 - 5368336
AN - SCOPUS:0014619555
SN - 0014-2956
VL - 11
SP - 456
EP - 464
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 3
ER -