Steroids in Germfree and Conventional Rats: Free Steroids in Faeces from Conventional Rats

H. Eriksson, J. Gustafsson, J. Sjövall

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16 Scopus citations

Abstract

Free steroids in faeces from germfree and conventional rats were studied by gas chromato‐graphy‐mass spectrometry after purification on carboxymethyl Sephadex LH‐20 and separation on methylated Sephadex G‐25. Only minor amounts of free steroids were found in germfree rats; these could not be identified. In contrast to this, conventional rats excreted considerable amounts of free steroids: 3α‐hydroxy‐5α,17α‐pregnan‐20‐one, 3α‐hydroxy‐5α‐pregnan‐20‐one, 3β‐hydroxy‐5α,17α‐pregnan‐20‐one (major component), 3β‐hydroxy‐5α‐pregnan‐20‐one, and 5α‐pregnane‐3β,20β‐diol. Furthermore, 5α‐pregnane‐3α,20β‐diol was tentatively identified. These findings are discussed in relation to what is known about steroid sulphates in faeces from germfree and conventional rats.

Original languageEnglish (US)
Pages (from-to)286-290
Number of pages5
JournalEuropean Journal of Biochemistry
Volume9
Issue number2
DOIs
StatePublished - Jun 1969

ASJC Scopus subject areas

  • Biochemistry

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