Steroids in Germfree and Conventional Rats: Free Steroids in Faeces from Conventional Rats

H. Eriksson, J. Gustafsson, J. Sjövall

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    28 Scopus citations

    Abstract

    Free steroids in faeces from germfree and conventional rats were studied by gas chromato‐graphy‐mass spectrometry after purification on carboxymethyl Sephadex LH‐20 and separation on methylated Sephadex G‐25. Only minor amounts of free steroids were found in germfree rats; these could not be identified. In contrast to this, conventional rats excreted considerable amounts of free steroids: 3α‐hydroxy‐5α,17α‐pregnan‐20‐one, 3α‐hydroxy‐5α‐pregnan‐20‐one, 3β‐hydroxy‐5α,17α‐pregnan‐20‐one (major component), 3β‐hydroxy‐5α‐pregnan‐20‐one, and 5α‐pregnane‐3β,20β‐diol. Furthermore, 5α‐pregnane‐3α,20β‐diol was tentatively identified. These findings are discussed in relation to what is known about steroid sulphates in faeces from germfree and conventional rats.

    Original languageEnglish (US)
    Pages (from-to)286-290
    Number of pages5
    JournalEuropean Journal of Biochemistry
    Volume9
    Issue number2
    DOIs
    StatePublished - Jun 1969

    ASJC Scopus subject areas

    • Biochemistry

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