Steroids in Germfree and Conventional Rats: 5. Identification of C19 Steroids in Faeces from Germfree Rats

J. Gustafsson, J. Sjövall

    Research output: Contribution to journalArticlepeer-review

    55 Scopus citations

    Abstract

    The C19 steroids in faeces from germfree rats have been studied by gas chromatography‐mass spectrometry. 3α,11β‐Dihydroxy‐5α‐androstan‐17‐one and 3α,7α‐dihydroxy‐5α‐androstan‐17‐one were the predominant steroids in this group and 5α‐androstane‐3α,7α,17β‐triol, 3α,19‐dihydroxy‐5α‐androstan‐17‐one were also identified. Evidence was obtained for the presence of 3α,15α‐dihydroxy‐5α‐androstan‐17‐one, 5α‐androstane‐3α,15α,17β‐triol and 5α‐androstane‐3α,17β‐diol. The latter compounds were present in small amounts and their stereochemistry could not be definitely established. The steroids were present in faeces as monosulphates as evidenced by their chromatographic behaviour on Sephadex LH‐20 and carboxymethyl Sephadex LH‐20. The possible significance of the predominance of 3α,5α steroid isomers in faeces from germfree rats is discussed.

    Original languageEnglish (US)
    Pages (from-to)227-235
    Number of pages9
    JournalEuropean Journal of Biochemistry
    Volume6
    Issue number2
    DOIs
    StatePublished - Nov 1968

    ASJC Scopus subject areas

    • Biochemistry

    Fingerprint

    Dive into the research topics of 'Steroids in Germfree and Conventional Rats: 5. Identification of C19 Steroids in Faeces from Germfree Rats'. Together they form a unique fingerprint.

    Cite this