Steroids in Germfree and Conventional Rats: 5. Identification of C19 Steroids in Faeces from Germfree Rats

J. Gustafsson, J. Sjövall

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

The C19 steroids in faeces from germfree rats have been studied by gas chromatography‐mass spectrometry. 3α,11β‐Dihydroxy‐5α‐androstan‐17‐one and 3α,7α‐dihydroxy‐5α‐androstan‐17‐one were the predominant steroids in this group and 5α‐androstane‐3α,7α,17β‐triol, 3α,19‐dihydroxy‐5α‐androstan‐17‐one were also identified. Evidence was obtained for the presence of 3α,15α‐dihydroxy‐5α‐androstan‐17‐one, 5α‐androstane‐3α,15α,17β‐triol and 5α‐androstane‐3α,17β‐diol. The latter compounds were present in small amounts and their stereochemistry could not be definitely established. The steroids were present in faeces as monosulphates as evidenced by their chromatographic behaviour on Sephadex LH‐20 and carboxymethyl Sephadex LH‐20. The possible significance of the predominance of 3α,5α steroid isomers in faeces from germfree rats is discussed.

Original languageEnglish (US)
Pages (from-to)227-235
Number of pages9
JournalEuropean Journal of Biochemistry
Volume6
Issue number2
DOIs
StatePublished - Nov 1968

ASJC Scopus subject areas

  • Biochemistry

Fingerprint

Dive into the research topics of 'Steroids in Germfree and Conventional Rats: 5. Identification of C<sub>19</sub> Steroids in Faeces from Germfree Rats'. Together they form a unique fingerprint.

Cite this