TY - JOUR
T1 - Steroids in Germfree and Conventional Rats
T2 - 3. Solvolyzable Sterol Conjugates in Germfree and Conventional Rat Faeces
AU - GUSTAFSSON, B. E.
AU - GUSTAFSSON, J.
AU - SJÖVALL, J.
PY - 1968/5
Y1 - 1968/5
N2 - A fraction of solvolyzable sterol conjugates having the chromatographic properties of steryl sulphates was isolated from faeces of germfree and conventional rats. The major sterols in this fraction were studied by gas‐liquid chromatography and gas chromatography‐mass spectrometry. Cholesterol, desmosterol, lathosterol, campesterol, methostenol, and β‐sitosterol were the predominant solvolyzable sterols in conventional animals, whereas cholesterol, desmosterol, and β‐sitosterol were the quantitatively most important sulphurylated sterols in germfree animals. The relative amounts of conjugated lathosterol was significantly higher in the conventional than in the germfree animals. Solvolyzable conjugates of sterols with a 3β, 5β‐configuration could not be found.
AB - A fraction of solvolyzable sterol conjugates having the chromatographic properties of steryl sulphates was isolated from faeces of germfree and conventional rats. The major sterols in this fraction were studied by gas‐liquid chromatography and gas chromatography‐mass spectrometry. Cholesterol, desmosterol, lathosterol, campesterol, methostenol, and β‐sitosterol were the predominant solvolyzable sterols in conventional animals, whereas cholesterol, desmosterol, and β‐sitosterol were the quantitatively most important sulphurylated sterols in germfree animals. The relative amounts of conjugated lathosterol was significantly higher in the conventional than in the germfree animals. Solvolyzable conjugates of sterols with a 3β, 5β‐configuration could not be found.
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U2 - 10.1111/j.1432-1033.1968.tb00251.x
DO - 10.1111/j.1432-1033.1968.tb00251.x
M3 - Article
C2 - 5660484
AN - SCOPUS:0014279060
SN - 0014-2956
VL - 4
SP - 574
EP - 577
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 4
ER -