TY - JOUR
T1 - Steroids in Germfree and Conventional Rats
T2 - 2. Identification of 3α‐,16α‐Dihydroxy‐5α‐Pregnan‐20‐One and Related Compounds in Faeces from Germfree Rats
AU - GUSTAFSSON, B. E.
AU - GUSTAFSSON, J.
AU - SJÖVALL, J.
PY - 1968/5
Y1 - 1968/5
N2 - Steroid conjugates having the chromatographic properties of steroid sulphates were isolated from faeces of germfree rats. After solvolysis and purification of the steroids by silicic acid chromatography the trimethylsilyl ethers and O‐methyl oxime‐trimethylsilyl ether derivatives were analyzed by combined gas chromatography‐mass spectrometry. 3α,16α‐Dihydroxy‐5α‐pregnan‐20‐one was identified as the predominant C21O3 steroid. Small amounts of 3β,16α‐dihydroxy‐pregn‐5‐en‐20‐one and another 16‐hydroxylated pregnanolone, tentatively identified as 3β,16α‐dihydroxy‐5α‐pregnan‐20‐one, were also found.
AB - Steroid conjugates having the chromatographic properties of steroid sulphates were isolated from faeces of germfree rats. After solvolysis and purification of the steroids by silicic acid chromatography the trimethylsilyl ethers and O‐methyl oxime‐trimethylsilyl ether derivatives were analyzed by combined gas chromatography‐mass spectrometry. 3α,16α‐Dihydroxy‐5α‐pregnan‐20‐one was identified as the predominant C21O3 steroid. Small amounts of 3β,16α‐dihydroxy‐pregn‐5‐en‐20‐one and another 16‐hydroxylated pregnanolone, tentatively identified as 3β,16α‐dihydroxy‐5α‐pregnan‐20‐one, were also found.
UR - https://www.scopus.com/pages/publications/0014281315
UR - https://www.scopus.com/inward/citedby.url?scp=0014281315&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1968.tb00250.x
DO - 10.1111/j.1432-1033.1968.tb00250.x
M3 - Article
C2 - 5660483
AN - SCOPUS:0014281315
SN - 0014-2956
VL - 4
SP - 568
EP - 573
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 4
ER -