Steroids in Germfree and Conventional Rats: 2. Identification of 3α‐,16α‐Dihydroxy‐5α‐Pregnan‐20‐One and Related Compounds in Faeces from Germfree Rats

B. E. GUSTAFSSON; J. GUSTAFSSON; J. SJÖVALL

doi:10.1111/j.1432-1033.1968.tb00250.x

Steroids in Germfree and Conventional Rats: 2. Identification of 3α‐,16α‐Dihydroxy‐5α‐Pregnan‐20‐One and Related Compounds in Faeces from Germfree Rats

B. E. GUSTAFSSON, J. GUSTAFSSON, J. SJÖVALL

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Abstract

Steroid conjugates having the chromatographic properties of steroid sulphates were isolated from faeces of germfree rats. After solvolysis and purification of the steroids by silicic acid chromatography the trimethylsilyl ethers and O‐methyl oxime‐trimethylsilyl ether derivatives were analyzed by combined gas chromatography‐mass spectrometry. 3α,16α‐Dihydroxy‐5α‐pregnan‐20‐one was identified as the predominant C₂₁O₃ steroid. Small amounts of 3β,16α‐dihydroxy‐pregn‐5‐en‐20‐one and another 16‐hydroxylated pregnanolone, tentatively identified as 3β,16α‐dihydroxy‐5α‐pregnan‐20‐one, were also found.

Original language	English (US)
Pages (from-to)	568-573
Number of pages	6
Journal	European Journal of Biochemistry
Volume	4
Issue number	4
DOIs	https://doi.org/10.1111/j.1432-1033.1968.tb00250.x
State	Published - May 1968

ASJC Scopus subject areas

Biochemistry

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10.1111/j.1432-1033.1968.tb00250.x

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title = "Steroids in Germfree and Conventional Rats: 2. Identification of 3α‐,16α‐Dihydroxy‐5α‐Pregnan‐20‐One and Related Compounds in Faeces from Germfree Rats",

abstract = "Steroid conjugates having the chromatographic properties of steroid sulphates were isolated from faeces of germfree rats. After solvolysis and purification of the steroids by silicic acid chromatography the trimethylsilyl ethers and O‐methyl oxime‐trimethylsilyl ether derivatives were analyzed by combined gas chromatography‐mass spectrometry. 3α,16α‐Dihydroxy‐5α‐pregnan‐20‐one was identified as the predominant C21O3 steroid. Small amounts of 3β,16α‐dihydroxy‐pregn‐5‐en‐20‐one and another 16‐hydroxylated pregnanolone, tentatively identified as 3β,16α‐dihydroxy‐5α‐pregnan‐20‐one, were also found.",

author = "GUSTAFSSON, \{B. E.\} and J. GUSTAFSSON and J. SJ{\"O}VALL",

year = "1968",

month = may,

doi = "10.1111/j.1432-1033.1968.tb00250.x",

language = "English (US)",

volume = "4",

pages = "568--573",

journal = "European Journal of Biochemistry",

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T2 - 2. Identification of 3α‐,16α‐Dihydroxy‐5α‐Pregnan‐20‐One and Related Compounds in Faeces from Germfree Rats

AU - GUSTAFSSON, B. E.

AU - GUSTAFSSON, J.

AU - SJÖVALL, J.

PY - 1968/5

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AB - Steroid conjugates having the chromatographic properties of steroid sulphates were isolated from faeces of germfree rats. After solvolysis and purification of the steroids by silicic acid chromatography the trimethylsilyl ethers and O‐methyl oxime‐trimethylsilyl ether derivatives were analyzed by combined gas chromatography‐mass spectrometry. 3α,16α‐Dihydroxy‐5α‐pregnan‐20‐one was identified as the predominant C21O3 steroid. Small amounts of 3β,16α‐dihydroxy‐pregn‐5‐en‐20‐one and another 16‐hydroxylated pregnanolone, tentatively identified as 3β,16α‐dihydroxy‐5α‐pregnan‐20‐one, were also found.

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Steroids in Germfree and Conventional Rats: 2. Identification of 3α‐,16α‐Dihydroxy‐5α‐Pregnan‐20‐One and Related Compounds in Faeces from Germfree Rats

Abstract

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