Steroid conjugates having the chromatographic properties of steroid sulphates were isolated from faeces of germfree rats. After solvolysis and purification of the steroids by silicic acid chromatography the trimethylsilyl ethers and O‐methyl oxime‐trimethylsilyl ether derivatives were analyzed by combined gas chromatography‐mass spectrometry. 3α,16α‐Dihydroxy‐5α‐pregnan‐20‐one was identified as the predominant C21O3 steroid. Small amounts of 3β,16α‐dihydroxy‐pregn‐5‐en‐20‐one and another 16‐hydroxylated pregnanolone, tentatively identified as 3β,16α‐dihydroxy‐5α‐pregnan‐20‐one, were also found.
|Original language||English (US)|
|Number of pages||6|
|Journal||European Journal of Biochemistry|
|State||Published - May 1968|
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