Steroids in Germfree and Conventional Rats: 2. Identification of 3α‐,16α‐Dihydroxy‐5α‐Pregnan‐20‐One and Related Compounds in Faeces from Germfree Rats

B. E. GUSTAFSSON, J. GUSTAFSSON, J. SJÖVALL

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Steroid conjugates having the chromatographic properties of steroid sulphates were isolated from faeces of germfree rats. After solvolysis and purification of the steroids by silicic acid chromatography the trimethylsilyl ethers and O‐methyl oxime‐trimethylsilyl ether derivatives were analyzed by combined gas chromatography‐mass spectrometry. 3α,16α‐Dihydroxy‐5α‐pregnan‐20‐one was identified as the predominant C21O3 steroid. Small amounts of 3β,16α‐dihydroxy‐pregn‐5‐en‐20‐one and another 16‐hydroxylated pregnanolone, tentatively identified as 3β,16α‐dihydroxy‐5α‐pregnan‐20‐one, were also found.

Original languageEnglish (US)
Pages (from-to)568-573
Number of pages6
JournalEuropean Journal of Biochemistry
Volume4
Issue number4
DOIs
StatePublished - May 1968

ASJC Scopus subject areas

  • Biochemistry

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