Steroid monosulfates in urine from male rat pseudohermaphrodites

The excretion of steroid monosulfates in urine from female, male and male pseudohermaphrodites of the same strain of King X-Holtzman rats was studied by gas chromatography—mass spectrometry. Female rats excreted C₁₉O₃ and C₂₁O₃ steroids of ovarian origin and C₂₁O₄ and C₂₁O₅ steroids of adrenal origin. No steroid monosulfates were found in urine from male rats. Male pseudohermaphrodites excreted C₁₉O₃ C₂₁O₄ and C₂₁O₅ steroids identical to the corresponding steroids in the female rat; however, no C₂₁O₃ steroids were found in urine from male pseudohermaphrodites. The present results show that the hepatic metabolism of steroids in male pseudohermaphrodites has a female character.