Steroid excretion patterns in urine from ovariectomized and adrenalectomized rats

Jan åke Gustafsson, Åke Pousette

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The steroid hormone metabolites in urine from adrenal- and gonadectomized female rats have been studied by gas chromatography-mass spectrometry. The C19 (3α, 7α-dihydroxy-5α-androstan-17-one, 3α, 19-dihydroxy-5α-androstan-17-one, 5α-androstane-3α, 7α, 17β-triol, and 3α, 11β-dihydroxy-5α-androstan-17-one), C21O2 (5α-pregnane-3α, 20β-diol) and C21O3 (3α, 19-dihydroxy-5α, 17α-pregnan-20-one, 3α, 17α-dihydroxy-5α, 17β-pregnan-20-one, 3α, 15α-dihydroxy-5α, 17β-pregnan-20-one, and 3α,16α-dihydroxy-5α, 17β-pregnan-20-one) steroid monosulphates in urine from female rats were of ovarian origin, since their excretion was not affected by adrenalectomy, whereas ovariectomy resulted in their disappearance from urine. On the other hand treatment of ovariectomized female rats with the 3β-hydroxy-Δ5-steroid oxidoreductase inhibitor 2α-cyano-4,4,17α-trimethyl-5-androsten-17β-ol-3-one resulted in the excretion of monosulphurylated 3β-hydroxy-5-androsten-17-one, 3β-hydroxy-5-pregnen-20-one, 3α, 16α-dihydroxy-5α, 17β-pregnan-20-one and 3β, 16α-dihydroxy-5-pregnen-20-one in urine. This shows that under abnormal conditions the adrenal can secrete C19 and 21-deoxy-C21 steroids, the metabolites of which are excreted in the urine.

Original languageEnglish (US)
Pages (from-to)182-186
Number of pages5
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume280
Issue number1
DOIs
StatePublished - Sep 7 1972

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

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