Staphylococcus pseudintermedius’s pbp4 is directly associated with the dissociated oxacillin and cefoxitin phenotype

Paula Gagetti, Roberto R. Rosato, Adriana E. Rosato

Research output: Contribution to journalArticlepeer-review

Abstract

Staphylococcus pseudintermedius is an important pathogen responsible for infections in dogs and in humans. The emergence and dissemination of methicillin-resistant S. pseudintermedius (MRSP) and the multidrug resistance frequently seen in this species make difficult the treatment of these pathogens. The cefoxitin disk is widely used as a marker of methicillin resistance mediated by the mecA gene in Staphylococcus aureus and other staphylococcal species; however, it is not useful to detect β-lactam resistance of MRSP in clinical microbiology laboratories. The purpose of this study was to elucidate the molecular bases of the dissociated phenotype between oxacillin and cefoxitin antibiotics. By using a combinatorial approach that included the Penicillin-Binding Proteins’ (PBP) profile, their affinity for different β-lactam antibiotics and the analyses of PBPs’ sequence, we provide evidence that PBP4 showed still affinity for its target cefoxitin, impairing its phenotypic resistant detection in MRSP. Together, these findings provide evidence that S. pseudintermedius PBP4 is directly associated with the dissociated oxacillin and cefoxitin phenotype.

Original languageEnglish (US)
Article number1299
JournalAntibiotics
Volume10
Issue number11
DOIs
StatePublished - Nov 2021

Keywords

  • Antimicrobial resistance
  • MRSP
  • Penicillin-Binding Proteins (PBPs)
  • S. pseudintermedius

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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