TY - JOUR
T1 - Stanniocalcin-1, an inhibitor of macrophage chemotaxis and chemokinesis
AU - Kanellis, John
AU - Bick, Roger
AU - Garcia, Gabriela
AU - Truong, Luan
AU - Tsao, Chun Chui
AU - Etemadmoghadam, Dariush
AU - Poindexter, Brian
AU - Feng, Lili
AU - Johnson, Richard J.
AU - Sheikh-Hamad, David
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2004/2
Y1 - 2004/2
N2 - In macrophages, changes in intracellular calcium have been associated with activation of cellular processes that regulate cell adhesion and motility and are important for the response of macrophages to antigenic stimuli. The mammalian counterpart of the fish calcium-regulating hormone stanniocalcin-1 (STC1) is expressed in multiple organs including the thymus and spleen, and hence, we hypothesized that it may have a role in modulating the immune/inflammatory response. Using murine macrophage-like (RAW264.7) and human monoblast-like (U937) cells to study chemotaxis in vitro, we found that STC1 attenuated chemokinesis and diminished the chemotactic response to monocyte chemotactic protein-1 (MCP-1) and stromal cell-derived factor-1α. Consistent with these findings, STC1 blunted the rise in intracellular calcium following MCP-1 stimulation in RAW264.7 cells. In vivo studies suggested differential expression of STC1 in obstructed kidney and localization to macrophages. MCP-1 and STC1 transcripts were both upregulated following ureteric obstruction, suggesting a functional association between the two genes. Our data suggest a role for mammalian STC1 in modulating the immune/ inflammatory response.
AB - In macrophages, changes in intracellular calcium have been associated with activation of cellular processes that regulate cell adhesion and motility and are important for the response of macrophages to antigenic stimuli. The mammalian counterpart of the fish calcium-regulating hormone stanniocalcin-1 (STC1) is expressed in multiple organs including the thymus and spleen, and hence, we hypothesized that it may have a role in modulating the immune/inflammatory response. Using murine macrophage-like (RAW264.7) and human monoblast-like (U937) cells to study chemotaxis in vitro, we found that STC1 attenuated chemokinesis and diminished the chemotactic response to monocyte chemotactic protein-1 (MCP-1) and stromal cell-derived factor-1α. Consistent with these findings, STC1 blunted the rise in intracellular calcium following MCP-1 stimulation in RAW264.7 cells. In vivo studies suggested differential expression of STC1 in obstructed kidney and localization to macrophages. MCP-1 and STC1 transcripts were both upregulated following ureteric obstruction, suggesting a functional association between the two genes. Our data suggest a role for mammalian STC1 in modulating the immune/ inflammatory response.
KW - Calcium homeostasis
KW - Kidney injury
KW - Monocyte chemotactic protein-1
KW - Stromal cell-derived factor-1α
KW - Ureteric obstruction
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U2 - 10.1152/ajprenal.00138.2003
DO - 10.1152/ajprenal.00138.2003
M3 - Article
C2 - 14570698
AN - SCOPUS:9144220759
VL - 286
SP - F356-F362
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
SN - 0363-6143
IS - 2 55-2
ER -