TY - JOUR
T1 - Standardization of T‐cell depletion in HLA matched bone marrow transplantation
AU - Gilmore, M. J.M.L.
AU - Patterson, J.
AU - Ivory, K.
AU - Brenner, M. K.
AU - Graphakos, S.
AU - Janossy, G.
AU - Hoffbrand, A. V.
AU - Prentice, H. G.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1986/9
Y1 - 1986/9
N2 - The IBM 2991 Blood Cell Processor has been used to isolate a mononuclear cell (MNC) fraction from the marrow of 31 allogeneic donors. The MNC fraction was then incubated with a combination of two murine monoclonal antibodies MBG6 (CD6) and RFT8 (CD8) followed by two rounds of treatment with rabbit complement resulting in a marrow inoculum significantly reduced in the number of T-lymphocytes. We report here new specifications for the use of Ficoll-Metrizoate, the method used to calculate T-lymphocyte depletion and the details of our attempts to improve T-depletion. Following marrow transplantation with this T-depleted fraction, 29 patients are evaluable for engraftment, one patient failed to engraft and one died too early for evaluation. Twenty-two had no acute graft-versus-host disease (aGvHD), at a minimum of 60 d, six had grade I acute GvHD and one grade III. No correlation was found between the absolute number of MNC infused and time to engraftment, nor any relationship between the number of residual viable T-lymphocytes in the infused marrow and the incidence of GvHD, but the patient with the most severe aGvHD also had the highest number of T-lymphocytes infused.
AB - The IBM 2991 Blood Cell Processor has been used to isolate a mononuclear cell (MNC) fraction from the marrow of 31 allogeneic donors. The MNC fraction was then incubated with a combination of two murine monoclonal antibodies MBG6 (CD6) and RFT8 (CD8) followed by two rounds of treatment with rabbit complement resulting in a marrow inoculum significantly reduced in the number of T-lymphocytes. We report here new specifications for the use of Ficoll-Metrizoate, the method used to calculate T-lymphocyte depletion and the details of our attempts to improve T-depletion. Following marrow transplantation with this T-depleted fraction, 29 patients are evaluable for engraftment, one patient failed to engraft and one died too early for evaluation. Twenty-two had no acute graft-versus-host disease (aGvHD), at a minimum of 60 d, six had grade I acute GvHD and one grade III. No correlation was found between the absolute number of MNC infused and time to engraftment, nor any relationship between the number of residual viable T-lymphocytes in the infused marrow and the incidence of GvHD, but the patient with the most severe aGvHD also had the highest number of T-lymphocytes infused.
UR - http://www.scopus.com/inward/record.url?scp=0022538285&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022538285&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2141.1986.tb07574.x
DO - 10.1111/j.1365-2141.1986.tb07574.x
M3 - Article
C2 - 3530316
AN - SCOPUS:0022538285
SN - 0007-1048
VL - 64
SP - 69
EP - 75
JO - British Journal of Haematology
JF - British Journal of Haematology
IS - 1
ER -