Stability of fibrin sealant in cerebrospinal fluid: An in vitro study

Tomas Menovsky, Joost De Vries, Marius Van den Bergh Weerman, J. André Grotenhuis, Robert G. Grossman, Allan H. Friedman, Philip E. Stieg, Howard A. Riina, William F. Chandler

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


OBJECTIVE: The in vitro stability of fibrin sealant in cerebrospinal fluid (CSF) was investigated to verify the efficacy of intracranial application of fibrin sealant. METHODS: Human CSF was collected from 11 patients. Fibrin glue spheres (diameter, 5 mm) were incubated in CSF specimens at 37°C. At 2, 4, 8, 24, and 48 hours, the diameter of the spheres was measured and the ultrastructure was evaluated by transmission electron microscopy. A control group consisted of sealant spheres in physiological saline (n = 2). RESULTS: In all CSF samples, the fibrin sealant did not degrade with time. The size, consistency, color, and shape of the sealant remained unchanged, even 48 hours after placement in the CSF. Transmission electron microscopic analysis of the fibrin sealant revealed an amorphous, fibrinous meshwork. No morphological differences existed between fibrin sealant complex placed in the CSF for different time periods and sealant placed in physiological saline. CONCLUSION: Within the limitations of this in vitro study, human CSF has no adverse effects on fibrin sealant in terms of alteration of structure and morphology. Fibrin sealant is stable in a CSF environment and can be effectively used in the cisternal or subarachnoidal space.

Original languageEnglish (US)
Pages (from-to)1453-1456
Number of pages4
Issue number6
StatePublished - Dec 1 2002
Externally publishedYes


  • Cerebrospinal fluid
  • Fibrin sealant
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery


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