Spectral and mobility measurements of single fluorescent nanobarcodes in submicrometer fluidic channels

S. M. Stavis; J. B. Edel; Y. Li; K. T. Samiee; D. Luo; Harold G. Craighead

Spectral and mobility measurements of single fluorescent nanobarcodes in submicrometer fluidic channels

S. M. Stavis, J. B. Edel, Y. Li, K. T. Samiee, D. Luo, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Fluorescent nanobarcodes have been developed by nucleic acid engineers to specifically and uniquely label biomolecules. Submicrometer fluidic channels were used to detect and identify individual nanobarcodes by their fluorescence emission and mobility. Two optical configurations were used. Focused 488 nm and 568 nm lasers were either overlapped or separated along the channel length to measure the spectral profile and speed of single nanobarcodes. Nanobarcodes were individually identifiable with varying degrees of accuracy.

Original language	English (US)
Title of host publication	Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Transducer Research Foundation
Pages	718-720
Number of pages	3
Volume	1
ISBN (Print)	0974361119, 9780974361116
State	Published - Jan 1 2005
Event	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States Duration: Oct 9 2005 → Oct 13 2005

Other

Other	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/Territory	United States
City	Boston, MA
Period	10/9/05 → 10/13/05

Keywords

Fluorescence
Nanobarcode
Nanofluidic
Single molecule

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Stavis, S. M., Edel, J. B., Li, Y., Samiee, K. T., Luo, D., & Craighead, H. G. (2005). Spectral and mobility measurements of single fluorescent nanobarcodes in submicrometer fluidic channels. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (Vol. 1, pp. 718-720). Transducer Research Foundation.

Spectral and mobility measurements of single fluorescent nanobarcodes in submicrometer fluidic channels. / Stavis, S. M.; Edel, J. B.; Li, Y. et al.

Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. p. 718-720.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Stavis, SM, Edel, JB, Li, Y, Samiee, KT, Luo, D & Craighead, HG 2005, Spectral and mobility measurements of single fluorescent nanobarcodes in submicrometer fluidic channels. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. vol. 1, Transducer Research Foundation, pp. 718-720, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 10/9/05.

Stavis SM, Edel JB, Li Y, Samiee KT, Luo D, Craighead HG. Spectral and mobility measurements of single fluorescent nanobarcodes in submicrometer fluidic channels. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1. Transducer Research Foundation. 2005. p. 718-720

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abstract = "Fluorescent nanobarcodes have been developed by nucleic acid engineers to specifically and uniquely label biomolecules. Submicrometer fluidic channels were used to detect and identify individual nanobarcodes by their fluorescence emission and mobility. Two optical configurations were used. Focused 488 nm and 568 nm lasers were either overlapped or separated along the channel length to measure the spectral profile and speed of single nanobarcodes. Nanobarcodes were individually identifiable with varying degrees of accuracy.",

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AU - Stavis, S. M.

AU - Edel, J. B.

AU - Li, Y.

AU - Samiee, K. T.

AU - Luo, D.

AU - Craighead, Harold G.

PY - 2005/1/1

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N2 - Fluorescent nanobarcodes have been developed by nucleic acid engineers to specifically and uniquely label biomolecules. Submicrometer fluidic channels were used to detect and identify individual nanobarcodes by their fluorescence emission and mobility. Two optical configurations were used. Focused 488 nm and 568 nm lasers were either overlapped or separated along the channel length to measure the spectral profile and speed of single nanobarcodes. Nanobarcodes were individually identifiable with varying degrees of accuracy.

AB - Fluorescent nanobarcodes have been developed by nucleic acid engineers to specifically and uniquely label biomolecules. Submicrometer fluidic channels were used to detect and identify individual nanobarcodes by their fluorescence emission and mobility. Two optical configurations were used. Focused 488 nm and 568 nm lasers were either overlapped or separated along the channel length to measure the spectral profile and speed of single nanobarcodes. Nanobarcodes were individually identifiable with varying degrees of accuracy.

KW - Fluorescence

KW - Nanobarcode

KW - Nanofluidic

KW - Single molecule

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M3 - Conference contribution

AN - SCOPUS:84902519328

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BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference

PB - Transducer Research Foundation

T2 - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005

Y2 - 9 October 2005 through 13 October 2005

ER -

Spectral and mobility measurements of single fluorescent nanobarcodes in submicrometer fluidic channels

Abstract

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Keywords

ASJC Scopus subject areas

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