Spectral and mobility measurements of single fluorescent nanobarcodes in submicrometer fluidic channels

S. M. Stavis, J. B. Edel, Y. Li, K. T. Samiee, D. Luo, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fluorescent nanobarcodes have been developed by nucleic acid engineers to specifically and uniquely label biomolecules. Submicrometer fluidic channels were used to detect and identify individual nanobarcodes by their fluorescence emission and mobility. Two optical configurations were used. Focused 488 nm and 568 nm lasers were either overlapped or separated along the channel length to measure the spectral profile and speed of single nanobarcodes. Nanobarcodes were individually identifiable with varying degrees of accuracy.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages718-720
Number of pages3
Volume1
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Keywords

  • Fluorescence
  • Nanobarcode
  • Nanofluidic
  • Single molecule

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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