TY - JOUR
T1 - Specific regulation of lipocalin-type prostaglandin D synthase in mouse heart by estrogen receptor β
AU - Otsuki, Michio
AU - Gao, Hui
AU - Dahlman-Wright, Karin
AU - Ohlsson, Claes
AU - Eguchi, Naomi
AU - Urade, Yoshihiro
AU - Gustafsson, Jan Åke
PY - 2003/9/1
Y1 - 2003/9/1
N2 - Estrogens have important physiological roles in the cardiovascular system. We use DNA microarray technology to study the molecular mechanism of estrogen action in the heart and to identify novel estrogen-regulated genes. In this investigation we identify genes that are regulated by chronic estrogen treatment of mouse heart. We present our detailed characterization of one of these genes, lipocalin-type prostaglandin D synthase (L-PGDS). Northern and Western blot analysis revealed that L-PGDS was induced both by acute and chronic estrogen treatment. Northern blot analysis, using estrogen receptor (ER)-disrupted mice, suggests that L-PGDS is specifically induced by ERβ in vivo. In further support of ERβ-selective regulation, we identify a functional estrogen-responsive element in the L-PGDS promoter, the activity of which is up-regulated by ERβ, but not by ERα. We demonstrate that a one-nucleotide change (A to C) in the L-PGDS estrogen-responsive element affects receptor selectivity.
AB - Estrogens have important physiological roles in the cardiovascular system. We use DNA microarray technology to study the molecular mechanism of estrogen action in the heart and to identify novel estrogen-regulated genes. In this investigation we identify genes that are regulated by chronic estrogen treatment of mouse heart. We present our detailed characterization of one of these genes, lipocalin-type prostaglandin D synthase (L-PGDS). Northern and Western blot analysis revealed that L-PGDS was induced both by acute and chronic estrogen treatment. Northern blot analysis, using estrogen receptor (ER)-disrupted mice, suggests that L-PGDS is specifically induced by ERβ in vivo. In further support of ERβ-selective regulation, we identify a functional estrogen-responsive element in the L-PGDS promoter, the activity of which is up-regulated by ERβ, but not by ERα. We demonstrate that a one-nucleotide change (A to C) in the L-PGDS estrogen-responsive element affects receptor selectivity.
UR - http://www.scopus.com/inward/record.url?scp=0043288096&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0043288096&partnerID=8YFLogxK
U2 - 10.1210/me.2003-0016
DO - 10.1210/me.2003-0016
M3 - Article
C2 - 12829806
AN - SCOPUS:0043288096
SN - 0888-8809
VL - 17
SP - 1844
EP - 1855
JO - Molecular Endocrinology
JF - Molecular Endocrinology
IS - 9
ER -