Specific regulation of lipocalin-type prostaglandin D synthase in mouse heart by estrogen receptor β

Michio Otsuki, Hui Gao, Karin Dahlman-Wright, Claes Ohlsson, Naomi Eguchi, Yoshihiro Urade, Jan Åke Gustafsson

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Estrogens have important physiological roles in the cardiovascular system. We use DNA microarray technology to study the molecular mechanism of estrogen action in the heart and to identify novel estrogen-regulated genes. In this investigation we identify genes that are regulated by chronic estrogen treatment of mouse heart. We present our detailed characterization of one of these genes, lipocalin-type prostaglandin D synthase (L-PGDS). Northern and Western blot analysis revealed that L-PGDS was induced both by acute and chronic estrogen treatment. Northern blot analysis, using estrogen receptor (ER)-disrupted mice, suggests that L-PGDS is specifically induced by ERβ in vivo. In further support of ERβ-selective regulation, we identify a functional estrogen-responsive element in the L-PGDS promoter, the activity of which is up-regulated by ERβ, but not by ERα. We demonstrate that a one-nucleotide change (A to C) in the L-PGDS estrogen-responsive element affects receptor selectivity.

Original languageEnglish (US)
Pages (from-to)1844-1855
Number of pages12
JournalMolecular Endocrinology
Volume17
Issue number9
DOIs
StatePublished - Sep 1 2003

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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